Analysis of Metabolic Characteristics of SIRT7 Low Expression Glioma Cell Line Using NMR-Based Metabonomics

doi:10.11938/cjmr20192774

Abstract

Abstract: Tumor is a metabolic disease. The effect of oncogene expression on the metabolism of cancer cells is one of the hotspots in cancer research. In this study, ¹H NMR-based metabonomics analysis was used to explore the metabolic characteristics of glioma cell lines with low expression of SIRT7, and to identify the characteristic metabolites and metabolic pathways related to the expression of SIRT7. The results showed that there were significant differences in metabolic profiles between the SIRT7 low expression group and the control group, and 22 aqueous metabolites were found to vary significantly. Compared with the control group, the concentration of 12 metabolites including lactate, glycine, and glutamate, and so on, increased in SIRT7 low expression glioma cell lines, while the concentration of 10 metabolites such as valine, leucine, lysine, et al. decreased. Pathway enrichment analysis indicated that the metabolic pathways of aminoacyl-tRNA biosynthesis, tyrosine metabolism, and so on, were closely related to the low expression of SIRT7. The results provide a theoretical basis for further mechanism elucidation of SIRT7 regulating glioma cell metabolism.

Key words: proton nuclear magnetic resonance, metabonomics, Sirtuin7, characteristic metabolites, metabolic pathways

CLC Number:

O482.53

SHAO Wei, LIN Qing-yuan, YANG Wen-sheng, HUANG Cai-hua, LIN Dong-hai. Analysis of Metabolic Characteristics of SIRT7 Low Expression Glioma Cell Line Using NMR-Based Metabonomics[J]. Chinese Journal of Magnetic Resonance, 2019, 36(4): 517-524.

References

[1] COLLER H A. Is cancer a metabolic disease[J]? Am J Pathol, 2014, 184(1):4-17.
[2] CHALKIADAKI A, GUARENTE L. The multifaceted functions of sirtuins in cancer[J]. Nat Rev Cancer, 2015, 15(10):608-624.
[3] VOELTER-MAHLKNECHT S, LETZEL S, MAHLKNECHT U. Fluorescence in situ hybridization and chromosomal organization of the human Sirtuin 7 gene[J]. Int J Oncol, 2006, 28(4):899-908.
[4] MICHISHITA E, PARK J Y, BURNESKIS J M, et al. Evolutionarily conserved and nonconserved cellular localizations and functions of human SIRT proteins[J]. Mol Biol Cell, 2005, 16(10):4623-4635.
[5] VAKHRUSHEVA O, SMOLKA C, GAJAWADA P, et al. Sirt7 increases stress resistance of cardiomyocytes and prevents apoptosis and inflammatory cardiomyopathy in mice[J]. Circ Res, 2008, 102(6):703-710.
[6] TONG Z, WANG Y, ZHANG X Y, et al. SIRT7 is activated by DNA and deacetylates histone H3 in the chromatin context[J]. ACS Chem Biol, 2016, 11(3):742-747.
[7] LI L, SHI L, YANG S D, et al. SIRT7 is a histone desuccinylase that functionally links to chromatin compaction and genome stability[J]. Nat Commun, 2016, 7:12235.
[8] LEE N, KIM D K, KIM E S, et al. Comparative interactomes of SIRT6 and SIRT7:Implication of functional links to aging[J]. Proteomics, 2014, 14(13,14):1610-1622.
[9] ZHANG S, CHEN P, HUANG Z A, et al. Sirt7 promotes gastric cancer growth and inhibits apoptosis by epigenetically inhibiting miR-34a[J]. Sci Rep, 2015, 5:9787.
[10] LI D, LI L F. MicroRNA3666 inhibits breast cancer cell proliferation by targeting sirtuin 7[J]. Mol Med Rep, 2017, 16(6):8493-8500.
[11] MU P F, LIU K, LIN Q Y, et al. Sirtuin 7 promotes glioma proliferation and invasion through activation of the ERK/STAT3 signaling pathway[J]. Oncol Lett, 2019, 17(2):1445-1452.
[12] SHAO W, GU J P, HUANG C H, et al. Metabolic profiles of CHG5 and U87 glioma cell lines derived by ¹H NMR[J]. Chinese J Magn Reson, 2014, 31(1):40-48. 邵巍, 顾金苹, 黄彩华, 等. NMR分析胶质瘤细胞系CHG5和U87的代谢轮廓[J]. 波谱学杂志, 2014, 31(1):40-48.
[13] COLEN C B, SHEN Y M, GHODDOUSSI F, et al. Metabolic targeting of lactate efflux by malignant glioma inhibits invasiveness and induces necrosis:an in vivo study[J]. Neoplasia, 2011, 13(7):620-632.
[14] SHAO W, GU J P, HUANG C H, et al. Malignancy-associated metabolic profiling of human glioma cell lines using ¹H NMR spectroscopy[J]. Mol Cancer, 2014, 13:197.
[15] LI T, LIU L B, WU H L, et al. Anti-herpes simplex virus type 1 activity of Houttuynoid A, a flavonoid from Houttuynia cordata Thunb[J]. Antiviral Res, 2017, 144:273-280.
[16] GOEMAN J J, VAN DE GEER S A, DE KORT F, et al. A global test for groups of genes:testing association with a clinical outcome[J]. Bioinformatics, 2004, 20:93-99
[17] XIA J, WISHART D S. MetPA:A web-based metabolomics tool for pathway analysis and visualization[J].Bioinformatics, 2010, 26(18):2342-2344
[18] SUN L H, FAN G L, SHAN P P, et al. Regulation of energy homeostasis by the ubiquitin-independent REGgamma proteasome[J]. Nat Commun, 2016, 7:12497.