An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation

doi:10.11938/cjmr20182622

Chinese Journal of Magnetic Resonance ›› 2019, Vol. 36 ›› Issue (2): 172-181.doi: 10.11938/cjmr20182622

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An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation

XIAO Xiong-jie^1,2,3, HU Mary³, ZHANG Xu¹, HU Jian-zhi³

1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan(Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. Pacific Northwest National Laboratory, Richland, WA 99352, USA

Received:2018-03-31 Published:2018-04-21
Supported by:
the National Science Foundation of China (21505153, 21120102038, 21675170, 81227902, 21475146, 21221064); Key Research Program of Frontier Sciences, CAS (QYZDJ-SSW-SLH027); the Award from National Institute of Environmental Health Sciences of the National Institute of Health (NIH) (R01ES022176).

Abstract

Abstract: The biological effects of ionizing radiation on organisms have emerged as a hot topic for research. In this study, the changes of metabolic profile in the kidney tissues extracts from C57BL/6J mice with or without exposing to whole body gamma or proton radiation were investigated with the liquid state proton nuclear magnetic resonance (¹H NMR)-based metabolomics approach. The results demonstrated that the exposed group had significantly different kidney metabolic profiles from the non-exposed group, involving mainly six metabolites, including 2-aminobutyrate, 3-hydroxybutyrate, homoserine, 1,3-diaminopropane, β-alanine and ascorbate. These metabolites could be used as potential biomarkers for the biological effects of high-dose ionizing radiation on kidney metabolism.

Key words: proton nuclear magnetic resonance (¹H NMR), metabolomics, ionizing radiation, kidney, multivariate data analysis

CLC Number:

O482.53

XIAO Xiong-jie, HU Mary, ZHANG Xu, HU Jian-zhi. An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation[J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 172-181.

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An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation

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