A 1H NMR-Based Metabonomic Study on the Serum of Haematitum-Treated Rats

doi:10.11938/cjmr20150408

Abstract

Abstract:

Wistar rats were intragastrically administered with different doses (0, 2, 5 g/kg body weight) of haematitum. ¹H NMR-based metabonomic techniques were used to analyze the metabolic profiles of the serum samples collected from the treated rats. Significant treatment-related changes were observed for the levels of β-hydroxybutyate, acetate, acetone, choline, glycerophosphorylcholine, glucose, lactate, low density lipoprotein, very low density lipoprotein and lipids. Such biochemical changes indicated that haematitum treatment at the dose of 2 or 5 g/kg body weight gave rise to reactive oxygen species (ROS) and caused peroxidation damage. It is concluded that haematitum treatment affects glucose, lipid and energy metabolism in rats.

Key words: nuclear magnetic resonance, metabonomics, principal component analysis, haematitum

CLC Number:

O482.53

WEI Ying1,2， ZHANG Wen-jun1， XUE Rong2， LI Xiao-jing2*， PEI Feng-kui2. A ¹H NMR-Based Metabonomic Study on the Serum of Haematitum-Treated Rats[J]. Chinese Journal of Magnetic Resonance, 2015, 32(4): 628-636.

References

[1] Chinese Pharmacopoeia Commission(国家药典委员会). Chinese Pharmacopoeia(中国药典)[M]. Beijing(北京): China Medical Science Press(中国医药科技出版社), 2012.

[2] Liu Shu-hua(刘淑花), Bi Jun-ying(毕俊英). The investigation of trace element and pharmacological action of ocher or calcined ocher (生或煅赭石微量元素含量及药理作用比较)[J]. Stud Trace Elem Health(微量元素与健康研究), 2003, 20(1): 6―7.

[3] Zheng Jian-han(郑建涵), Wu Zhen-hua(吴振华). Discussoin of best ways of processnig ruddle(中药代赭石最佳炮制方法探讨)[J]. CATCM(中医药学刊), 2006, 24(8): 1 559―1 560.

[4] Liu Dan(刘丹), Li Jun-song(李俊松), Li Wei-dong(李伟东), et al. Studies on changes of content of eleven elements in crude and calcined ochre(赭石炮制前后 11 种元素的含量变化研究)[J]. CATCM(中华中医药学刊), 2008, 26(12): 2 577―2 578.

[5] Li Hong-liang(李红亮). The study of Fu-xuan-dai-zhe-tang in treatment of post-stroke hiccup(旋覆代赭汤治疗中风后呃逆浅析)[J]. World Chinese Med(世界中医药), 2013, 8(1): 65―66.

[6] Nicholson J K, Lindon J C, Holmes E. 'Metabonomics': understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data [J]. Xenobiotica, 1999, 29(11): 1 181―1 189.

[7] Duarte I F, Diaz S O, Gil A M. NMR metabolomics of human blood and urine in disease research[J]. J Pharmaceut Biomed, 2014, 93(S1): 17―26.

[8] Liu S, Wang W, Zhou X Y, et al. Dose responsive effects of cisplatin in L02 cells using NMR-based metabolomics[J]. Environ Toxicol Phar, 2014, 37(1): 150―157.

[9] Li M H, Wang J S, Lu Z G, et al. NMR-based metabolomics approach to study the toxicity of lambda-cyhalothrin to goldfish (Carassius auratus)[J]. Aquat Toxicol, 2014, 146: 82―92.

[10] Anni L, Riikka J, Jari S, et al. Postprandial response on fatty meal is affected by sea buckthorn (Hippophaë rhamnoides) supplementation: NMR metabolomics study[J]. Food Res Int, 2014, 58: 23―34.

[11] Ernst M, Silva D B, Silva R R, et al. Mass spectrometry in plant metabolomics strategies: from analytical platforms to data acquisition and processing[J]. Nat prod Rep, 2014, 31 (6): 784―806.

[12] Feng J H, Zhao J, Tang H R, et al. NMR-based metabonomic analyses of the effects of ultrasmall superparamagnetic particles of iron oxide (USPIO) on macrophage metabolism[J]. J Nanopart Res, 2011, 13(5): 2 049―2 062.

[13] Qiu Yu-jie(邱玉洁), Xia Sheng-an (夏圣安), Liu Mai-li(刘买利), et al. Pattern recognition methods in biomedical magnetic resonance(生物医学核磁共振中的模式识别方法)[J]. Chinese J Magn Reson(波谱学杂志), 2005, 22(1): 99―111.

[14] Zhang Pan-pan(张盼盼), Zhang Wen-jun(章文军), Li Xiao-jing(李晓晶), et al. Studies on the serum of arsenic trionxide treated rats by 1H NMR based metabonomics(基于 1H NMR 的代谢组学方法对大鼠砒霜给药后血清的研究)[J]. Chinese J Anal Chem (分析化学), 2013, 41(9): 1 434―1 438.

[15] Wei L, Liao P Q, Li X J, et al. Metabolic profiling studies on the toxicological effects of realgar in rats by 1H NMR spectroscopy[J]. Toxicol Appl Pharm, 2009, 234(3): 314―325.

[16] Dong Ji-yang(董继扬), Xu Le(徐乐), Xu Jing-jing(许晶晶), et al. Adaptive binning method for NMR spectroscopic metabonmics data preprocessing(核磁共振代谢组学数据处理中的自适应分段积分方法)[J]. Chem J of Chinese U(高等学校化学学报), 2009, 30(6): 1 101―1 108.

[17] Zheng X F, Tian J S, Liu P, et al. Analysis of the restorative effect of Bu-zhong-yi-qi-tang in the spleen-qi deficiency rat model using 1H NMR-based metabonomics[J]. J Ethnopharmacol, 2014, 151(2): 912―920.

[18] Eaton J W, Qian M. Molecular bases of cellular iron toxicity[J]. Free Radical Bio Med, 2002, 32(9): 833―840.

[19] Hershko C, Link G, Ioav C. Pathophysiology of iron over load[J]. Ann NY Acad Sci, 1998, 850(1): 191―201.

[20] Bacon B R, Tavill A S, Brittenham G M, et al. Hepatic lipid peroxidation in vivo in rats with chronic iron overload[J]. J Clin Invest, 1983, 71(3): 429―439.

[21] Warburg O, Wind F, Negelein E. The metabolism of tumors in the body[J]. J Gen Physiol, 1927, 8(6): 519―530.

[22] McGarry J D, Foster D W. Regulation of hepatic fatty acid oxidation and ketone body production[J]. Annu Rev Biochem, 1980, 49: 395―420.

[23] Knowles S E, Jarrett I G, Filsell O H, et al. Production and utilization of acetate in mammals[J]. Biochem J, 1974, 142(2): 401―411.

[24] Zeisel S H. Dietary choline: biochemistry, physiology, and pharmacology[J]. Annu Rev Nutr, 1981, 1(1): 95―121.

[25] Griffin J L, Mann C J, Scott J, et al. Choline containing metabolites during cell transfection: an insight into magnetic resonance spectroscopy detectable changes[J]. FEBS Lett, 2001, 509(2): 263―266.

[26] Peters T J, Selden C, Seymour C A. Lysosomal disruption in the pathogenesis of hepatic damage in primary and secondary haemochromatosis[J]. Ciba Found Symp, 1976, 51: 317―329.

A ¹H NMR-Based Metabonomic Study on the Serum of Haematitum-Treated Rats

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	HU Kun, SUN Han-dong, PUNO Pema-tenzin. Application of Quantum Chemical Calculation of Nuclear Magnetic Resonance Parameters in the Structure Elucidation of Natural Products [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 359-376.
[2]	WANG Hong-zhi, WANG Shen-lin, HU Bin-wen, YU Yi-hua, SONG Yi-qiao, YAO Ye-feng. A Virtual NMR Spectrometer Based on Numerical Computational Simulations [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 288-297.
[3]	LIU Zhi-jun, YANG Dong, SHAO Ji-xi, HU Yao-qing. Evolution of Pore Connectivity in the Fushun Oil Shale by Low-Field Nuclear Magnetic Resonance Spectroscopy [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 309-318.
[4]	YIN Tian-peng, WANG Ya-rong, WANG Min, SHI Wen-zhi, ZHANG Zheng-qian, HE Sha-sha. Complete Assignments of NMR Spectral Data of Three C₁₉-Diterpenoid Alkaloids [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 331-340.
[5]	YANG Yun-han, DU Yao, YING Fei-xiang, YANG Jun-li, XIA Da-zhen, XIA Fu-ting, YANG Li-juan. Inclusion Behavior of Naringenin/β-Cyclodextrin Supramolecular Complex [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 319-330.
[6]	WANG Ya-lan, WANG Xiao-jing, WANG Zhi-wei. Spectral Analyses and Structural Elucidation of Azilsartan [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 350-358.
[7]	LIU Ji-hong, JIN Kun, WANG Ping, LUO Gen. An NMR Study on Esculetin and It's Derivatives [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 341-349.
[8]	CAO Yuan, WU Yong-ping, CHEN Dong-jun. A Spectroscopic Study on Tautomerism of Selaginellins from Selaginella [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 155-163.
[9]	CHEN Xiao-ying, YU Gang-jin, MAO Shi-zhen, LIU Mai-li, DU You-ru. Mixing-Induced Decreases in Critical Micelle Concentration in Aqueous Solution of Surfactants:Probing into the Mechanisms with ¹H NMR Spectroscopy [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 219-224.
[10]	XIAO Xiong-jie, HU Mary, ZHANG Xu, HU Jian-zhi. An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 172-181.
[11]	KOU Xin-hui, LIU Yi-xiang, LIU Xing-hong, LI Cong-gang, LIU Mai-li, JIANG Ling. Visualizing the Pre-Active Conformation of Response Regulator PhoB_N^F20D in Its apo State [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 164-171.
[12]	WANG Shuang-hong, ZENG Pei, PAN Si-na, WANG Jia, WANG Shu-mei, YANG Yong-xia. Effects of Gegen Qinlian Decoction on the Fecal Metabonome of High Fructose-Induced Insulin Resistance Rats Studied by ¹H NMR Spectroscopy [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 182-194.
[13]	LI Ying-jun, YANG Kai-dong, JIN Kun, LIU Ji-hong, WANG Si-yuan, ZHANG Nan. NMR Study of A Novel Carbazole-Isatin Based Bis-Thiocarbohydrozone Derivative [J]. Chinese Journal of Magnetic Resonance, 2019, 36(1): 83-92.
[14]	CHI Xiu-juan, QIAO Xiao-ya, LIU Ying, LIU Hui-li, CHEN Lei, WANG Ji-hui, AI Xuan-jun. Purification of the AtGrp7 RRM Domain from Arabidopsis thaliana and Its Preliminary Structure and Binding Analysis [J]. Chinese Journal of Magnetic Resonance, 2019, 36(1): 1-14.
[15]	YIN Tian-peng, LUO Zhi-hui, CAI Le, DING Zhong-tao. Research Progress and NMR Spectral Features of Natural C₁₉-Diterpenoid Alkaloids [J]. Chinese Journal of Magnetic Resonance, 2019, 36(1): 113-126.