Plasma Metabonomics in Tuberculosis Patients at Different Stages

doi:10.11938/cjmr20160205

Abstract

Abstract:

Tuberculosis (TB) is the second largest infectious disease caused by a single pathogen leading to mortality, only after AIDS. Discovery of biomarkers associated with disease progression is vital for TB screening and control. Metabonomics is among the most useful tools for this purpose. A few metabonomic studies on TB patients have been published. In this study, the plasma samples of TB patients with varying severity were studied by ¹H NMR based metabonomics. Orthogonal partial least squares-discriminant analysis (OPLS-DA) models showed that TB patients at different stages and healthy controls could be differentiated by their plasma metabolic profiles. It was found that the TB groups had significantly higher levels of valine, alanine, creatine, and 3-hydroxybutyrate, relative to the healthy controls. In the TB patients, the levels of lactate, pyruvate, N-acetyl glycoprotein, leucine and glutamate increased gradually with disease progression, while the level of citrate, a tricarboxylic acid (TCA) cycle intermediate, decreased with disease progression. The plasma metabolic profile changes indicated increased energy metabolism and glycolysis, enhanced ketogenesis of fatty acid, reduced TCA cycle, and disordered amino acid metabolism in the TB patients.

Key words: NMR, metabonomics, OPLS-DA, tuberculosis, differential metabolites

CLC Number:

O482.53

ZHOU Ning, DU Xiang-bo, YANG Li, XU Yi, SUN Bo, ZHANG Qi, DAI Er-hei, YAN Xian-zhong, DONG Fang-ting. Plasma Metabonomics in Tuberculosis Patients at Different Stages[J]. Chinese Journal of Magnetic Resonance, 2016, 33(2): 224-235.

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