波谱学杂志 ›› 2021, Vol. 38 ›› Issue (4): 448-459.doi: 10.11938/cjmr20212929
收稿日期:
2021-06-29
出版日期:
2021-12-05
发布日期:
2021-08-10
通讯作者:
陈忠
E-mail:chenz@xmu.edu.cn
基金资助:
Xiao-qing LIN,Shi-jia DU,Hao-lin ZHAN,Yu-qing HUANG,Zhong CHEN*()
Received:
2021-06-29
Online:
2021-12-05
Published:
2021-08-10
Contact:
Zhong CHEN
E-mail:chenz@xmu.edu.cn
摘要:
二维J分解(2D JRES)核磁共振(NMR)波谱是一种简单且用户友好的谱图表达形式,其将J偶合常数和化学位移信息分离到两个正交的频率维度上.自40年前首次被提出以来,2D JRES技术在脉冲序列和方法的改进,以及实际应用的进展方面一直备受关注.本文回顾了新型2D JRES脉冲序列,以及用于精确测量同核J偶合常数的2D J编辑谱的最近进展,特别是基于纯化学位移演化机制的正交相敏2D JRES谱方法及其应用,并阐述其在克服强偶合效应和磁场不均匀性等方面的能力.
中图分类号:
林晓晴,杜世佳,詹昊霖,黄玉清,陈忠. 基于纯化学位移的同核二维正交相敏J分解核磁共振波谱[J]. 波谱学杂志, 2021, 38(4): 448-459.
Xiao-qing LIN,Shi-jia DU,Hao-lin ZHAN,Yu-qing HUANG,Zhong CHEN. Two-Dimensional Homonuclear Orthogonal-Pattern Phase-Sensitive J-Resolved NMR Spectroscopy Based on Pure Shifts[J]. Chinese Journal of Magnetic Resonance, 2021, 38(4): 448-459.
图3
基于PSYCHE的正交相敏2D JRES谱—RASA2DJ. (a) 脉冲序列,由1D PSYCHE和回波链J采样模块组成.在第一个非选择性π/2脉冲之前适用了一个预饱和(Presat)模块进行水峰抑制.长条形脉冲分别代表π/2和π脉冲,两个带有斜箭头的半圆形代表小翻转角(β < < π/2)的扫频方向相反的脉冲,G1和G2是相干选择梯度,G3是与扫频脉冲配合使用的弱梯度,t1是间接维演化时间,t2是直接维采样时间,SW1是t1对应的谱宽,T是相邻两个π脉冲之间的间隔.(b)以二维相敏谱中的单峰信号为例展示数据相敏处理流程,包括关于F1'=0轴翻转原始谱图后,将其与原始谱图相加,并将相加后的谱图沿F2'维进行一维相位校正可得相敏结果(摘自文献[44])
图7
应用于不均匀磁场下的高分辨2D J-编辑谱方法. (a)基于3D采样的HR-G-SERF实验序列,相位循环:Φ1 (x, -x), Φ2 (x), Φ3 (x, x, y, y, -x, -x, -y, -y), Φrec (x, -x, -x, x). (b)基于2D快速采样的AH-G-SERF实验序列,由ZS模块和G-SERF回波链模块组成设计的,相位循环:Φ1=Φ2=Φ3=Φrec=x,Φ4在J编辑回波链采样模块中以(x, x, x, x, -x, -x, -x, -x)N/2为循环.细绿色长条和粗绿色长条分别表示π/2和π非选择性脉冲,蓝色和红色填充形状表示的分别是用于选择特定质子和空间编码的π选择性脉冲;Gz为空间编码的弱梯度,G1、G2、G3和G4为相干选择梯度(摘自文献[53])
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