Myocardial ischemia with different durations (0 min, 5 min, 20 min and 45 min) was induced in adult male SD rats. The myocardium was then harvested, divided into three parts (the infarcted region, the border zone and the normal appearing region), frozen with liquid nitrogen, pulverized and extracted with perchloric acid (PCA). The extracts obtained were lyophilized, re-dissolved in ²H₂O and analyzed by ³¹P MRS. The results show that, in the infarcted region, the PCr/Pi ratio decreased to 12 % of control after 5 min ischemia; the ATP/Pi ratio decreased to 0.05 and the Pi/EPP ratio increased to 0.8 after 20 min ischemia; the PDE/ATP ratio reached 1.93 after 45 min ischemia. The normal region also showed metabolite changes. The PCr/Pi ratio after 5 min ischemia and the ATP/Pi ratio after 45 min ischemia decreased to 20 % and 43 % of those in the normal appearing region respectively. Conclusions: (1) ³¹P MRS on PCA extracts can be used to study energy and phospholipids metabolism in myocardium; (2) The normal appearing region showed metabolite changes, suggesting that energy reserve and utilization decreased in this region; (3) The PDE/ATP ratio may be used as a marker for irreversible ischemic injuries in rat myocardium.

In this study, MRI and localized in vivo ¹H-MRS were applied to study cerebral changes during the early stage of a rat model of chronic temporal epilepsy. Epilepsy was induced by repetitive tetanus to the right basal dendrite region. Six days after the initial tetanus, T₂-weighted MRI (T-₂W-MRI) revealed that there was a significant increase in signal intensity of the right ventral part of hippocampus, the signal intensity of the same region decreased significantly in diffusion-weighted MRI (DWI). Such changes may be caused by the increase of the ratio of free water to bounded water in the affected region. Decrease of NAA was not found in the foci of epilepsy with localized in vivo ¹H-MRS, suggesting that there existed little or no loss of neurons during the early stage of this model of temporal epilepsy.

To investigate the feasibility of using ¹⁹F-NMR spectroscopy to monitor cytosine deaminase (CD) transgene expression in human colon cancer cell line. The SW1116 cell line was transfected with a gene coding for CD from-E.coli- by the retroviral mediated method. The assessment of the expression of CD gene in the colon cancer cell line was carried out by RT-PCR, MTT assay of drug sensitivity to 5-FC and ¹⁹F-NMR spectroscopy. The results indicate that the transgenic SWCD₂ cell line was more sensitive to the prodrug 5-fluorcytosine (5-FC) (IC₅₀=66 μmol/L) as compared to the parent cancer cells SW1116 (IC₅₀=16 mmol/L). After incubation of the cultured CD⁺ cells in 774 μmol/L 5-FC for 24 h, ¹⁹F-NMR spectra of the intact cells showed two broad resonances corresponding to 5-FC (taken up into cell chemical shift relative to trifluoroacetate of δ-91.7) and 5-FC produced by the action of CD (chemical shif of δ-93.3), respectively. These results demonstrated the feasibility of using ¹⁹F-NMR spectroscopy to noninvasively monitor CD gene expression in human colon cancer cells. This capability provides a new approach for measuring gene expression that will be useful in clinical gene therapy trials.

This paper presents a detection method for NQR explosive based on the Apollo signal controller. The principles, component parts, and operating method of the system were discussed. The system was tested using RDX samples. The results show that RDX signals can be detected by this method.

The structures of seven higher carbon sugar Schiff’s bases were elucidated. The complete assignment of ¹H and ¹³C NMR chemical shifts of these 7 compounds was obtained by 2D NMR methods, including ¹H-¹H COSY, HMQC and HMBC.

Two pairs of stereo-isomers with germacranolide type, sabertopin and isoscabertopin, and isodeoxyelephantopin and deoxyelephantopin, were isolated from Elephantopus scaber. Their ¹H chemical shifts were assigned correctly by the NOESY technique. The two protons signals of C-3 in the stereo-isomers were found to be in opposite positions, and so were the two protons signals of C-9.

NMR spectroscopy was used to study a potential antibacterial agent, 1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7-(4-phenylacetylaminothiocarbamoyl-1-piperazinyl)-3-quinolinecarboxylic acid. The ¹H and ¹³C NMR chemical shifts of the compound were assigned using ¹]H-¹H COSY, gHMBC and attached proton test (APT)  experiments.

A new diterpenoid, 19-methoxy-scalarin, was isolated from marine sponge Hyrtios erectus. Its structure was determined by 1D and 2D NMR spectroscopy. In combination with the “excitation sculpting” technique, 1D GOESY was used for its configuration determination. The experimental results show that, compared to the commonly used NOE difference method, 1D GOESY provides a more direct and easy way for configuration determination.

The procedures in the chemical reaction involving fluorine-containing lactones were studied using two dimensional nuclear magnetic resonance techniques. The structures of the reaction intermediates and products were determined by ¹H NMR,

There are many ways of design for the magnets used in nuclear magnetic resonance. However,  it is always challenging to make the magnet small, light, stable and having large volume with uniform magnetic field. Solution to this challenge lies in accurate computation of the electromagnetism field. In this paper, we first briefly reviewed the problems in magnet design, and then showed how to optimize the different ways of magnet design. An optimized magnet design was proposed, based on which a portable NMR cock analyzer was built successfully. The results from a number of on site tests showed that the portable NMR rock analyzer we built has high stability and good reproducibility, and is suitable for working under severe conditions.

The rubidium spectral lamp is one of the important parts of a gas cell rubidium atomic frequency standard (RAFS). It has great influences on the performance of the RAFS. To achieve high stability and meet the needs of working in high environmental temperature and vacuum conditions, a new type of the spectral lamp has been designed and developed. The stability of the RF oscillator circuit, the electric screening of the RF coil and thermal property of the bulb and its housing were taken into account in the design.

¹H-Indene-5-carboxamine, N-dibutyl-2,3-dihydro-2-(3-hydroxy-6-(1-methylethy)-2-quinolinyl)-1,3-dioxo is a widely used yellow D₂T₂ dye. In this paper, the ¹H and ¹³C NMR chemical shifts of the dye were assigned and its structures were determined. The influence of its structure in CDCl₃ on its chemical shifts was also discussed.

Long chain phosphonic yelides were hydrolyzed under suitable conditions. The reaction products were studied by ¹]H NMR, ¹³C NMR, IR, MS and microanalysis. A possible mechanism of the hydrolysis reaction was proposed.

Having the potential of being used as α₁-adrenoceptor antagonist and new antihypertensive drugs, ten phenylpiperazine derivatives were synthesized, and their structures were determined by ¹H NMR, ¹³C NMR, HMBC, HSQC and ¹H-¹H DQF-COSY. The ¹H and ¹³C chemical shifts of these compounds were assigned. The relationship between the substitute groups and the ¹³C chemical shifts was discussed.

This article reviewed the principles of TROSY and CRINEPT, and their applications in the fields of proteomics, structural biology, structural genomics, protein structure determination and studies of interactions between molecules. Because they can suppress the fast transverse relaxation effect effectively and enhance the magnetization transfer efficiencies respectively, TROSY and CRINEPT play especially important roles in the protein structure determination when the molecular weight of the protein is large than 30 kDa.