Chinese Journal of Magnetic Resonance ›› 2004, Vol. 21 ›› Issue (3): 371-383.

Previous Articles    

TWO EFFECTIVE METHODS FOR  IMPROVING NMR SENSITIVITY AND RESOLUTION IN STUDIES OF BIOMACROMOLECULES: TROSY AND  CRINEPT

 ZHOU Zhi-Ming1,2, LIU Mai-Li2*, ZHANG Xu2, XIE Chao-Hui2   

  1. 1. Department of Chemistry, HuaZhong University of Science & Technology, Wuhan 430074, China; 
    2.State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics (Wuhan Institute of Physics  and Mathematics,  Chinese Academy of Sciences), Wuhan 430071, China
  • Received:2004-01-12 Revised:2004-03-11 Online:2004-09-05 Published:2004-09-05
  • Supported by:

    科技部重大基础研究计划(973,2002CB713806), 国家自然科学基金资助项目(NSFC,20175035).

Abstract:

This article reviewed the principles of TROSY and CRINEPT, and their applications in the fields of proteomics, structural biology, structural genomics, protein structure determination and studies of interactions between molecules. Because they can suppress the fast transverse relaxation effect effectively and enhance the magnetization transfer efficiencies respectively, TROSY and CRINEPT play especially important roles in the protein structure determination when the molecular weight of the protein is large than 30 kDa.

Key words: NMR, TROSY, review article, CRINEPT, 2D NMR

CLC Number: