Chinese Journal of Magnetic Resonance ›› 2015, Vol. 32 ›› Issue (4): 648-660.doi: 10.11938/cjmr20150410

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The Distribution of Metabolites in Different Root Parts from Tree Peony

XIAO Chao-niWANG PeiMA Cui-xiaRONG Ze-mingZHENG Xiao-hui*
  

  1. College of Life Sciences, Northwest University, Xi’an 710069, China
  • Received:2015-10-16 Revised:2015-11-09 Online:2015-12-05 Published:2015-12-05
  • About author:*Corresponding author: ZHENG Xiao-hui, Tel: +86-29-88302686, E-mail: zhengxh@nwu.edu.cn.
  • Supported by:

    陕西省重点科技创新团队资助项目(2013KCT-24)

Abstract:

The distribution of metabolites in the root bark of tree peony from different root parts was systematically analyzed using the combination of NMR and HPLC-MS for the first time. A total of 16 metabolites from their methanol extracts were simultaneously identified and quantified, including 1 primary metabolite (sucrose) and 15 secondary ones such as 4 acetophenones, 6 phenolics, 2 monoterpene glycosides, 1 flavonoid and 2 unsaturated fatty acids. The quantitative data indicated that there are the higher levels of acetophenones and sucrose, and the lower contents of phenolics, monoterpene glycosides, flavonoids and unsaturated fatty acids in the tree peony roots. NMR-based metabolomics revealed that the metabolite compositions were found to be different among the three root parts. The increasing levels of acetophenones and decreasing level of sucrose were observed in second lateral root, the first lateral and axial root in order. The axial roots have higher quality than the lateral roots in tree peony due to the accumulation of bioactive secondary metabolites associated with plant physiology. These findings provided important scientific evidence for grading the root bark of tree peony on the general market.

Key words: NMR, HPLC-MS, metabolite, root part, tree peony

CLC Number: