Probing Membrane Protein Interactions by 19F Solid-State NMR

doi:10.11938/cjmr20182618

Abstract

Abstract: The ¹⁹F nucleus has a high gyromagnetic ratio that is comparable to that of proton. Due to its high sensitivity and low background in the biological systems, ¹⁹F labeling has emerged as an attractive approach in biological solid-state NMR studies. In this review, we summarized the widely used ¹⁹F-labeling strategies, ¹⁹F solid-state NMR spectroscopy techniques to probe membrane protein interactions, and the recent applications of ¹⁹F solid-state NMR in characterizing of membrane proteins. The limitations of ¹⁹F solid-state NMR were also highlighted.

Key words: ¹⁹F solid-state NMR, protein interaction, review, membrane protein

CLC Number:

O482.53

XU Xiao-jun, WANG Shen-lin. Probing Membrane Protein Interactions by ¹⁹F Solid-State NMR[J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 238-251.

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Probing Membrane Protein Interactions by ¹⁹F Solid-State NMR

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