The Distribution of Metabolites in Different Root Parts from Tree Peony

doi:10.11938/cjmr20150410

Abstract

Abstract:

The distribution of metabolites in the root bark of tree peony from different root parts was systematically analyzed using the combination of NMR and HPLC-MS for the first time. A total of 16 metabolites from their methanol extracts were simultaneously identified and quantified, including 1 primary metabolite (sucrose) and 15 secondary ones such as 4 acetophenones, 6 phenolics, 2 monoterpene glycosides, 1 flavonoid and 2 unsaturated fatty acids. The quantitative data indicated that there are the higher levels of acetophenones and sucrose, and the lower contents of phenolics, monoterpene glycosides, flavonoids and unsaturated fatty acids in the tree peony roots. NMR-based metabolomics revealed that the metabolite compositions were found to be different among the three root parts. The increasing levels of acetophenones and decreasing level of sucrose were observed in second lateral root, the first lateral and axial root in order. The axial roots have higher quality than the lateral roots in tree peony due to the accumulation of bioactive secondary metabolites associated with plant physiology. These findings provided important scientific evidence for grading the root bark of tree peony on the general market.

Key words: NMR, HPLC-MS, metabolite, root part, tree peony

CLC Number:

O482.53

XIAO Chao-ni， WANG Pei， MA Cui-xia， RONG Ze-ming， ZHENG Xiao-hui*. The Distribution of Metabolites in Different Root Parts from Tree Peony
[J]. Chinese Journal of Magnetic Resonance, 2015, 32(4): 648-660.

References

[1] Pharmacopoeia Commission of People’s Republic of China(国家药典委员会). Pharmacopoeia of People’s Republic of China (中华人民共和国药典)[M]. Beijing(北京): Chemical Industry Press(化学工业出版社), 2010.

[2] Hu S L, Shen G, Zhao W G, et al. Paeonol, the main active principles of Paeonia moutan, ameliorates alcoholic steatohepatitis in mice[J]. J Ethnopharmacol, 2010, 128(1): 100－106.

[3] Fu P K, Yang C Y, Tsai T H, et al. Moutan cortex radicis improves lipopolysaccharide-induced acute lung injury in rats through anti-inflammation[J]. Phytomedicine, 2012, 19(13): 1 206－1 215.

[4] Yun C S, Choi Y G, Jeong M Y, et al. Moutan cortex radicis inhibits inflammatory changes of gene expression in lipopolysaccharide-stimulated gingival fibroblasts[J]. J Nat Med, 2013, 67(3): 576－589.

[5] Park J, Kim H Y, Lee S M. Protective effects of Moutan cortex radicis against acute hepatotoxicity[J]. Afr J Tradit Complem Alterna Med, 2011, 8(5): 220－225.

[6] Kim H G, Park G, Piao Y, et al. Effects of the root bark of Paeonia suffruticosa on mitochondria-mediated neuroprotection in an MPTP-induced model of Parkinson's disease[J]. Food Chem Toxicol, 2014, 65: 293－300.

[7] He C N, Peng Y, Zhang Y C, et al. Phytochemical and biological studies of Paeoniaceae[J]. Chem Biodivers, 2010, 7: 805－838.

[8] Zha L P, Cheng M E, Peng H S. Identification of ages and determination of paeoniflorin in roots of Paeonia lactiflora Pall. From four producing areas based on growth rings[J]. Microsc Res Techniq, 2012, 75(9): 1 191－1 196.

[9] Ding Y, Wu E Q, Chen J B, et al. Quality evaluation of Moutan cortex radicis using multiple component analysis by high performance liquid chromatography[J]. B Kor Chem Soc, 2009, 30(10): 2 240－2 244.

[10] Long Quan-jiang(龙全江), Wang Xiao-ge(王晓阁), Zhou Zhou(周宙), et al. Study on the influence of the contents of paeonol in the cutting process of fresh Cortex moutan(趁鲜切制法对牡丹皮饮片中丹皮酚含量的影响研究)[J]. Journal of Chinese Medicinal Materials(中药材), 2012, 35(6): 883－886.

[11] Sun Qing-lei(孙庆雷), Zhao Hong-xia(赵红霞), Lin Yun-liang(林云良), et al. ¹H NMR fringerprints of Scutellaria Baicalensis(黄芩的¹H NMR指纹图谱研究)[J]. Chinese J Magn Reson(波谱学杂志), 2007, 24(2): 163－168.

[12] Chen Bo(陈波), Kang Hai-ning(康海宁), Han Chao(韩超), et al. Applications of NMR spectroscopy and pattern recognition in food analysis (NMR指纹图谱与模式识别方法在食物分析中的应用)[J]. Chinese J Magn Reson(波谱学杂志), 2006, 23(3): 397－407.

[13] Dai H, Xiao C N, Liu H B, et al. Combined NMR and LC-MS analysis reveals the metabonomic changes in Salvia miltiorrhiza Bunge induced by water depletion[J]. J Proteome Res, 2010, 9: 1 460－1 475.

[14] Herve Du P C, Imberty A, Roques N, et al. Conformational behavior of sucrose and its deoxy analogue in water as determined by NMR and molecular modeling[J]. J Am Chem Soc, 1991, 113(10): 3 720－3 727.

[15] Kwon D Y, Song H N, Yoon S H. Synthesis of medium-chain glycerides by lipase in organic solvent[J]. J Am Oil Chem Soc, 1996, 73(11): 1 521－1 525.

[16] Kuwajima H, Shibano N, Baba T, et al. An acetophenone glycoside from Exacum affine[J]. Phytochemistry, 1996, 41(1): 289－292.

[17] Yu J, Lang H Y, Xiao P G. A new compound, apiopaeonoside, isolated from the root of Paeonia suffruticosa[J]. Acta Pharm Sin, 1986, 21(3): 191－197.

[18] Ha D T, Trung T N, HienT T, et al.Selected compounds derived from Moutan Cortex stimulated glucose uptake and glycogen synthesis via AMPK activation in human HepG2 cells[J]. J Ethnopharmacol, 2010, 131(2): 417－424.

[19] Peungvicha P, Temsiririrkkul R, Prasain J K, et al. 4-Hydroxybenzoic acid: a hypoglycemic constituent of aqueous extract of Pandanus odorus root[J]. J Ethnopharmacol, 1998, 62(1): 79－84.

[20] Lee S C, Kwon Y S, Son K H, et al. Antioxidative constituents from Paeonia lactiflora[J]. Arch Pharm Res, 2005, 28(7): 775－783.

[21] Puppala M, Ponder J, Suryanarayana P, et al. The isolation and characterization of beta-glucogallin as a novel aldose reductase inhibitor from Emblica officinalis[J]. Plos One, 2012, 7(4): e31399.

[22] Beretta G, Artali R, Caneva E, et al. Conformation of the tridimensional structure of 1, 2, 3, 4, 6-pentagalloyl-beta-D- glucopyranose (PGG) by H-1 NMR, NOESY and theoretical study and membrane interaction in a simulated phospholipid bilayer: a first insight[J]. Magn Reson Chem, 2010, 49(3): 132－136.

[23] Piao X S, Piao X L, Kim H Y, et al. Antioxidative activity of geranium (Pelargonium inquinans Ait) and its active component, 1, 2, 3, 4, 6-penta-O-galloyl-beta-D-glucose[J]. Phytother Res, 2008, 22(4): 534－538.

[24] Qi S H,Wu D G, Ma Y B, et al. A novel flavane carapa guianensis[J]. Acta Bot Sin, 2013, 45(9): 1 129－1 133.

[25] Drew S W, Demain A L. Effect of primary metabolites on secondary metabolism[J]. Annu Rev Microbiol, 1977, 31: 343－356.

[26] Wang X, Cheng C, Sun Q L, et al. Isolation and purification of four flavonoid constituents from the flowers of Paeonia suffruticosa by high-speed counter-current chromatography[J]. J Chromatogra A, 2005, 1 075(1-2): 127－131.

[27] Li C, Du H, Wang L, et al. Flavonoid composition and antioxidant activity of tree peony (Paeonia Section Moutan) yellow flowers[J]. J Agric Food Chem, 2009, 57(18): 8 496－8 503.

[28] Baetz U, Martinoia E. Root exudates: the hidden part of plant defense[J]. Trends Plant Sci, 2013, 19(2): 90－98.