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Sequence Development and Parameter Optimization for A Pseudo-Continuous Arterial Spin Labeling Method on 1.5 T Clinical Magnetic Resonance Imaging Systems
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WEI Qing1,2,SONG Rui-bo2,ZHOU Xiao-dong2,TANG Wei-jun4,ZHANG Wei-guo3,CHEN Qun1*
Chinese Journal of Magnetic Resonance, 2015, 32(4): 574-583.
DOI: 10.11938/cjmr20150403
Pseudo-continuous arterial spin labeling (pCASL) is perfusion imaging technique that combines the advantages of continuous arterial spin labeling (CASL) and pulsed arterial spin labeling (PASL). In this study, we implemented a pCASL sequence on uMR560 1.5 T MRI systems developed by United Imaging Healthcare Cooperation. Experiments were performed to demonstrate the stability of the pCASL sequence implemented and the accuracy of perfusion quantification. The tagging efficiency was first measured through phantom experiments. In order to achieve the highest signal-to-noise ratio, post-labeling delay time and labeling distance were optimized empirically to be approximately 70 mm blow the imaging slab and 1 200 ms, respectively. Lastly, perfusion-weighted signal intensity and absolute cerebral blood flow (CBF) were measured from 12 normal volunteers using the pCASL sequence implemented, with satisfactory results obtained from 10 subjects. In summary, the pCASL sequence implemented on the uMR560 1.5 T MRI systems can be used for perfusion imaging on human brain, with reasonable stability and accuracy.
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A 1 H NMR-Based Metabonomic Study on the Serum of Haematitum-Treated Rats
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WEI Ying1,2, ZHANG Wen-jun1, XUE Rong2, LI Xiao-jing2*, PEI Feng-kui2
Chinese Journal of Magnetic Resonance, 2015, 32(4): 628-636.
DOI: 10.11938/cjmr20150408
Wistar rats were intragastrically administered with different doses (0, 2, 5 g/kg body weight) of haematitum. 1 H NMR-based metabonomic techniques were used to analyze the metabolic profiles of the serum samples collected from the treated rats. Significant treatment-related changes were observed for the levels of β -hydroxybutyate, acetate, acetone, choline, glycerophosphorylcholine, glucose, lactate, low density lipoprotein, very low density lipoprotein and lipids. Such biochemical changes indicated that haematitum treatment at the dose of 2 or 5 g/kg body weight gave rise to reactive oxygen species (ROS) and caused peroxidation damage. It is concluded that haematitum treatment affects glucose, lipid and energy metabolism in rats.
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Characterization of Molecular Structure of DAST Using Nuclear Magnetic Resonance Spectroscopy
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PANG Zi-bo*, MENG Da-lei, DOU Ying, MA Si-rui, WU Cong,CHENG Hong-juan, XU Yong-kuan
Chinese Journal of Magnetic Resonance, 2015, 32(4): 637-647.
DOI: 10.11938/cjmr20150409
DAST crystal (4-N , N -dimethylamino-4’-N’ -methyl-stilbazolium tosylate crystal) shows electro-optical effect, and has excellent properties for second-order nonlinear optics. It can be used for wave radiation and detection from infrared to terahertz range. In this study, the molecular structure and purity of DAST crystal were analyzed by liquid-state NMR spectroscopy (i.e., 1 H NMR, 13 C NMR, 1 H-1 H COSY, HSQC and HMBC) using CD3 OD and DMSO-d 6 as solvents. It was observed that DAST tended to exist in the form of ion pair in CD3 OD solution, while in the form of dissociative state in DMSO-d 6 solution. This could be due to solvent-dependent redistribution of electron cloud in the conjugated structure and movement of the framework.
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The Distribution of Metabolites in Different Root Parts from Tree Peony
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XIAO Chao-ni, WANG Pei, MA Cui-xia, RONG Ze-ming, ZHENG Xiao-hui*
Chinese Journal of Magnetic Resonance, 2015, 32(4): 648-660.
DOI: 10.11938/cjmr20150410
The distribution of metabolites in the root bark of tree peony from different root parts was systematically analyzed using the combination of NMR and HPLC-MS for the first time. A total of 16 metabolites from their methanol extracts were simultaneously identified and quantified, including 1 primary metabolite (sucrose) and 15 secondary ones such as 4 acetophenones, 6 phenolics, 2 monoterpene glycosides, 1 flavonoid and 2 unsaturated fatty acids. The quantitative data indicated that there are the higher levels of acetophenones and sucrose, and the lower contents of phenolics, monoterpene glycosides, flavonoids and unsaturated fatty acids in the tree peony roots. NMR-based metabolomics revealed that the metabolite compositions were found to be different among the three root parts. The increasing levels of acetophenones and decreasing level of sucrose were observed in second lateral root, the first lateral and axial root in order. The axial roots have higher quality than the lateral roots in tree peony due to the accumulation of bioactive secondary metabolites associated with plant physiology. These findings provided important scientific evidence for grading the root bark of tree peony on the general market.
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A Mini NMR Sensor for Measuring Water Content inside Large Timber
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GUO Pan1, JI Yong-liang2, HE Wei1, XU Zheng1*
Chinese Journal of Magnetic Resonance, 2015, 32(4): 678-687.
DOI: 10.11938/cjmr20150413
The content and distribution of water in wood affect its performance. So far, most technologies to measure water content/distribution can only be applied to small wood samples. A method to measure the water content inside large timber is urgently needed, given that information on water content/distribution inside the harvested timber is often required before the timber can be processed to products. In this work, a mini cylindrical NMR sensor (i.e., 28 mm in diameter and 60 mm in length) for measuring water content inside large timber was designed. The sensitive volume is a coaxial ring just outside of the sensor. For measurement, the sensor needs to be placed inside a hole drilled on the timber, which has a similar diameter with the sensor. By moving the sensor around the wall of the hole, the NMR parameters of the timber could be measured. Preliminary experimental results showed that the sensor can detect signals from both free water and the bound water in large timber, and such signals can be used to calculate relative water contents.
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NMR Technologies for Evaluating Oil & Gas Shale: A Review
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WANG Zhi-zhan1*, LI Xin1, WEI Yang-xu2, QIN Li-ming1, LU Huang-sheng1
Chinese Journal of Magnetic Resonance, 2015, 32(4): 688-698.
DOI: 10.11938/cjmr20150414
For conventional reservoir, NMR is an effective technology that can be used to evaluate petrophysics and pore structure, distribution and saturation of pore fluid rapidly, nondestructively and economically. Applying NMR technologies to evaluate shale reservoir, however, often encounters problems such as low resolution and inapplicable interpretation and evaluation models, due to reservoir characteristics such as nanoscale pore, complex mineral composition, complex pore structure, high organic content, ultralow permeability, influence of internal field gradients and restricted diffusion et al. In this paper, we reviewed recent progresses in NMR technologies for shale oil and gas exploration and exploitation, and related data analysis methods. Special attention was given to areas such as improvement of NMR detection resolution and novel evaluation models for pore structure, rock physical property, and pore fluid distribution and recognition. Two areas needed further research are identified: relaxation mechanisms inside nano-pore and improving pore scale resolution of D -T 2 . Petrophysics evaluation model and pore fluid evaluation model also need to be further optimized.