Chinese Journal of Magnetic Resonance

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In Vitro and In Vivo Evaluation of Tumor-Targeting Efficacy of Folate-Conjugated Magnetic Albumin Nanospheres by MRI

TANG Qiu-sha1*,AN Yan-li2,YANG Rui1   

  1. 1. Department of Pathology and Pathophysiology, Southeast University, Nanjing 210009, China; 
    2. Jiangsu Key Laboratory of Molecular Imaging and Functional Imaging, Medical School of Southeast University, Nanjing 210009, China
  • Received:2013-06-18 Revised:2013-07-16 Online:2013-12-05 Published:2013-12-05
  • About author:*Corresponding author: Tang Qiu-sha,Tel: 025-83272373,E-mail: panyixi-tqs@163.com.
  • Supported by:

    国家自然科学基金资助项目(81071881).

Abstract:

This study evaluated the tumor-targeting efficacy of folate-conjugated magnetic albumin nanospheres with in vitro and in vivo MRI experiments. Human nasopharyngeal epidermal KB cells were used for the in vitro experiment, which were incubated with folate-conjugated and non-folate-conjugated nanospheres for 24 hours. The presence of the nanospheres inside the cells were then detected using MRI and  Prussian blue staining. For the in vivo experiment, nude mice were transplanted subcutaneously with nasopharyngeal epidermal KB tumor. MRI and histopathological examination were used to detect the presence of nanospheres in the tumors after injection. The in vitro and in vivo experiments demonstrated that folate-conjugated nanospheres had significantly higher amount of deposition in the tumor cells/tumors than the non-folate-conjugated nanospheres. It was concluded that folate-conjugated magnetic albumin nanospheres had a better targeting efficacy on human nasopharyngeal epidermal KB cells, relative to the non-folate-conjugated nanospheres.

Key words: magnetic resonance imaging, targeting imaging, folate, cancer

CLC Number: