NMR Analysis of Metabonomic Fingerprints and Footprints of HepG2 Cells

Abstract

Abstract:

Analyzing metabolic features is an important approach to understand biochemistry of cells. In this work, NMR spectroscopy was employed to analyze the metabolite compositions of HepG2 cells and culture media. More than 50 metabolites were detected and unambiguously assigned based on ¹H and ¹³C NMR data. These metabolites are known to be involved in metabolic pathways such as tricarboxylic acid cycle (TCA), gluconeogenesis, glycolysis, metabolisms of amino acids, fatty acids, choline, purines and pyrimidines. Metabolomes of the cells and their culture media were shown to offer complementary information on cellular metabolic “fingerprints” and “footprints”. With NMRbased metabonomic approaches, the metabolic effects of quercetin, a plant secondary metabolite, on HepG2 cells were studied. The results of this work demonstrated that NMR is an efficient tool for analyzing cellular metabolome and its responses to drug interventions.

Key words: Nuclear Magnetic Resonance (NMR), metabonomics, quercetin, HepG2 cell

CLC Number:

O482.53

YAO Jian-wu1,2，AN Yan-peng1，LONG Jin1，SHI Xiao-huo1，TANG Hui-ru1*. NMR Analysis of Metabonomic Fingerprints and Footprints of HepG2 Cells[J]. Chinese Journal of Magnetic Resonance.

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