Methanol-Induced Oxidative Stress in Mice Studied by Electron Paramagnetic Resonance

Abstract

Abstract:

A mouse model of acute methanol poisoning was established to evaluate the protective effects of rhubarb and vitamin C (Vc) on methanol-induced oxidative stress in different organs. The mice used in the study were randomly divided into five group: blank group, control group, methanol group, rhubarb group and Vc group. EPR spin trapping technique was employed to study the intensity change of spin adducts induced by reactive oxygen species (ROS) free radicals generated in different organs of the mice. Compared with the control group, the animals in the methanol group showed significantly increased amount of ROS free radicals in the organs, suggesting the induction of acute oxidative stress. Rhubarb and Vc were shown to be able to alleviate acute methanolinduced oxidative stress.

Key words: EPR, oxidative stress, spin trapping, mice, methanol, free radical

CLC Number:

O657.61

ZHOU Li-fang1，ZHU Xiang1，ZHAO Hong-li1，LAN Min-bo1,2*，SHI Xin-mei2. Methanol-Induced Oxidative Stress in Mice Studied by Electron Paramagnetic Resonance[J]. Chinese Journal of Magnetic Resonance.

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