Abstract: Teniposide is a semisynthetic podophyllotoxin obtainable from the extracts of the roots and the rhizomes of May apple or mandrake plant Podophyllum peltatum. As an anticancer agent, Teniposide has become a major chemotherapy drug for the treatment of intracranial malignant tumor. In this study, the ¹³C and ¹H chemical shifts of the compound were assigned using 1D NMR and 2D NMR techniques including COSY, TOCOSY, NOESY, gHSQC and gHMBC. Its structure was determined from the NMR data.

Key words: NMR, assignments, 2D NMR, teniposide, anti-tumor drug