基于磁共振的胞浆中无标记酵母细胞色素c构象变化追踪

doi:10.11938/cjmr20222985

波谱学杂志 ›› 2023, Vol. 40 ›› Issue (1): 22-29.doi: 10.11938/cjmr20222985

基于磁共振的胞浆中无标记酵母细胞色素c构象变化追踪

占建华^1,²,胡琴^1,²,朱勤俊¹,蒋滨^1,^2,^3,⁴,张许^1,^2,^3,^4,^#(),刘买利^1,^2,^3,^4,^*()

1.波谱与原子分子物理国家重点实验室，武汉磁共振中心（中国科学院精密测量科学与技术创新研究院），湖北武汉 430071
2.中国科学院大学，北京 100049
3.华中科技大学，武汉光电国家研究中心，湖北武汉 430074
4.湖北光谷实验室，湖北武汉 430074

收稿日期:2022-03-21 出版日期:2023-03-05 在线发表日期:2022-04-28
通讯作者: 张许,刘买利 E-mail:zhangxu@wipm.ac.cn;ml.liu@wipm.ac.cn.
基金资助:
国家重点基础研究发展计划资助项目(2017YFA0505400);国家重点基础研究发展计划资助项目(2018YFE0202300);国家重点基础研究发展计划资助项目(2018YFA0704002)

Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR

ZHAN Jianhua^1,²,HU Qin^1,²,ZHU Qinjun¹,JIANG Bin^1,^2,^3,⁴,ZHANG Xu^1,^2,^3,^4,^#(),LIU Maili^1,^2,^3,^4,^*()

1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan (Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences), Wuhan 430071, China
2. University of Chinese Academy of Sciences, Beijing 100049, China
3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
4. Optics Valley Laboratory, Wuhan 430074, China

Received:2022-03-21 Published:2023-03-05 Online:2022-04-28
Contact: ZHANG Xu,LIU Maili E-mail:zhangxu@wipm.ac.cn;ml.liu@wipm.ac.cn.

摘要/Abstract

摘要：

细胞色素c（cytochrome c，cyt c）是一个重要的多功能蛋白. 在线粒体中，它作为载体传递电子. 而在细胞质中，它可能会作为凋亡起始因子启动细胞凋亡程序. 复杂的细胞质环境是否会对其构象产生影响，以及产生怎样的影响，目前仍然没有得到确证. 本研究通过无标记的基于甲基的核磁共振（nuclear magnetic resonance，NMR）技术追踪了野生型酿酒酵母iso-1 cyt c在酵母细胞匀浆液中的构象变化. 发现cyt c在细胞匀浆中至少存在4种不同的氧化态构象和1种还原态构象. 而且随着时间的推进，不同构象之间发生转换. 结果表明cyt c的构象会随环境改变，这可能与抵抗氧化应激相关.

关键词: 核磁共振, 构象变化, 甲基, 细胞色素c

Abstract:

Cytochrome c (cyt c) is an important multifunctional protein. In mitochondria, it acts as a carrier for electron transporting. In cytoplasm, it may act as an apoptotic initiator to initiate the apoptotic process. Whether and how a complex cytoplasmic environment affects its conformation has not been confirmed yet. In this study, the conformational changes of wild type saccharomyces cerevisiae iso-1 cyt c in yeast cell homogenate were tracked by methyl-based nuclear magnetic resonance (NMR) technique. At least four different oxidative conformations and one reduced conformation of cyt c were identified in cell homogenate. And over time, the transitions among different conformations of cyt c were observed. The results indicate that the conformation of cyt c changes with environment, which may be closely related to resistance to oxidative stress.

Key words: nuclear magnetic resonance (NMR), conformational change, methyl, cytochrome c

中图分类号:

O482.53

占建华,胡琴,朱勤俊,蒋滨,张许,刘买利. 基于磁共振的胞浆中无标记酵母细胞色素c构象变化追踪[J]. 波谱学杂志, 2023, 40(1): 22-29.

ZHAN Jianhua,HU Qin,ZHU Qinjun,JIANG Bin,ZHANG Xu,LIU Maili. Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR[J]. Chinese Journal of Magnetic Resonance, 2023, 40(1): 22-29.

图/表 4

参考文献 29

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基于磁共振的胞浆中无标记酵母细胞色素c构象变化追踪

Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR

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