HR-MAS 1H NMR Spectra of Rat Kidney Tissue Analyzed by Statistical Total Correlation Spectroscopy (STOCSY)

Abstract

Abstract:

Providing spectral resolution comparable to those of high resolution spectroscopy on tissue extracts, high resolution magic angle spinning (HR-MAS) NMR spectroscopy on intact tissue has been used widely in NMR-based metabonomic studies. In order to prevent enzymatic degradation and/or diffusion of metabolites, the HR-MAS NMR experiments are often performed at low temperature with a short acquisition time. Statistical total correlation spectroscopy (STOCSY) is a novel tool developed previously for NMR-based metabonomic studies. It takes advantage of the multicollinearity of the intensity variables in a set of ¹H NMR spectra to generate a pseudo-two-dimensional NMR spectrum, without needing extra acquisition time. In this paper, STOCSY was used to analyze HR-MAS ¹H spectra of rat kidney tissue. Pseudo 2D spectra were generated from a set of 1D HR-MAS NMR spectra and used to observe intramolecular correlations among the resonances. The STOCSY results are helpful in peak assignment.

Key words: NMR, statistical total correlation spectroscopy (STOCSY), magic angle spinning, tissue, kidneys

CLC Number:

O482.53

LI Zhao1,2， ZHU Hang1,2，CHENG Peng1,2，ZHOU Zhi-ming1,2，LIU Mai-li1，ZHANG Xu1*. HR-MAS ¹H NMR Spectra of Rat Kidney Tissue Analyzed by Statistical Total Correlation Spectroscopy (STOCSY)[J]. Chinese Journal of Magnetic Resonance, 2007, 24(4): 510-518.

References

[1] Tang H R, Wang Y L. Metabonomics: A revolution in progress[J]. Prog Biochem Biophys, 2006, 33(5): 401-417.

[2] Lindon J C, Holmes E, Nicholson J K. Recent development and applications of NMR-based metabonomics[J]. Chinese J Magn Reson(波谱学杂志), 2006, 23(1): 101-127.

[3] Nicholson J K, Lindon J C, Holmes E. ´Metabonomics´: understanding the metabolic responses of living systems to pathophysiological stimuli via multivariate statistical analysis of biological NMR spectroscopic data[J]. Xenobiotica, 1999, 29(11): 1 181-1 189.

[4] Constantinou M, Papakonstantinou E, Spraul M, et al. ¹H MNR-based metabonomics for the diagnosis of inborn errors of metabolism in urine[J]. Anal Chim Acta, 2005, 542: 169-177.

[5] Constantinou M, Papakonstantinou E, Benaki D, et al. Application of nuclear magnetic resonance spectroscopy combined with principal component analysis in detecting inborn errors of metabolism using blood Spots. A metabonomic approach[J]. Anal Chim Acta, 2004, 511: 303-312.

[6] Moolenaar S H, Engelke U F, Wevers R A. Proton nuclear magnetic resonance spectroscopy of body fluids in the field of inborn errors of metabolism[J]. Ann Clin Biochem, 2003, 40(Pt 1): 16-24.

[7] Lindon J C, Keun H C, Ebbels T M, et al. The consortium for metabonomic toxicology (COMET): aims, activities and achievements[J]. Pharmacogenomics, 2005, 6(7): 691-699.

[8] Lindon J C, Nicholson J K, Holmes E, et al. Contemporary issues in toxicology the role of metabonomics in toxicology and its evaluation by the COMET project[J]. Toxicol Appl Pharm, 2003, 187(3): 137-146.

[9] Bundy J G, Spurgeon D J, Svendsen C, et al. Earthworm species of the genus Eisenia can be phenotypically differentiated by metabolic profiling[J]. FEBS Lett, 2002, 521(1-3): 115-120.

[10] Bundy J G, Lenz E M, Bailey N J, et al. Metabonomic assessment of toxicity of 4-fluoroaniline, 3,5-difluoroaniline and 2-fluoro-4-methylaniline to the earthworm Eisenia veneta (Rosa): identification of new endogenous biomarkers[J]. Environ Toxicol Chem, 2002, 21(9): 1 966-1 972.

[11] Zhu Hang（朱航）, Tang Hui-ru（唐惠儒）, Zhang Xu（张许）, et al. NMR-based metabonomics(基于NMR的代谢组学研究)[J]. Hua Xue Tong Bao(化学通报), 2006, 69: 1-9.

[12] Yan Xian-zhong(颜贤忠), Zhao Jian-yu(赵剑宇), Peng Shuang-qing(彭双清), et al. Metabonomics in post-genomic era(代谢组学在后基因组时代的作用)[J]. Chinese J Magn Reson(波谱学杂志), 2004, 21(2): 263-271.

[13] Vejchapipat P, Williams S R, Spitz L, et al. Intestinal metabolism after ischemia-reperfusion[J]. J Pediatr Surg, 2000, 35(5): 759-764.

[14] Cheng L L, Chang I W, Smith B L, et al. Evaluating human breast ductal carcinomas with high-resolution magic-angle spinning proton magnetic resonance spectroscopy[J]. J Magn Reson, 1998, 135(1): 194-202.

[15] Duarte I F, Stanley E G, Holmes E, et al. Metabolic assessment of human liver transplants from biopsy samples at the donor and recipient stages using high-resolution magic angle spinning ¹H NMR spectroscopy[J]. Anal Chem, 2005, 77(17): 5 570-5 578.

[16] Barton S J, Howe F A, Tomlins A M, et al. Comparison of in vivo ¹H MRS of human brain tumours with ¹H HR-MAS spectroscopy of intact biopsy samples in vitro[J]. Magma, 1999, 8(2): 121-128.

[17] Griffin J L, Williams H J, Sang E, et al. Abnormal lipid profile of dystrophic cardiac tissue as demonstrated by one- and two-dimensional magic-angle spinning ¹H NMR spectroscopy[J]. Magn Reson Med, 2001, 46(2): 249-255.

[18] Waters N J, Holmes E, Waterfield C J, et al. NMR and pattern recognition studies on liver extracts and intact livers from rats treated with alpha-naphthylisothiocyanate[J]. Biochem Pharmacol, 2002, 64(1): 67-77.

[19] Garrod S, Humpher E, Connor S C, et al. High-resolution ¹H NMR and magic angle spinning NMR spectroscopic investigation of the biochemical effects of 2-bromoethanamine in intact renal and hepatic tissue\[J\]. Magn Reson Med, 2001, 45(5): 781-790.

[20] Garrod S, Humpfer E, Spraul M, et al. High-resolution magic angle spinning ¹H NMR spectroscopic studies on intact rat renal cortex and medulla[J]. Magn Reson Med, 1999, 41(6): 1 108-1 118.

[21] Moka D, Vorreuther R, Schicha H, et al. Biochemical classification of kidney carcinoma biopsy samples using magic-angle-spinning ¹H nuclear magnetic resonance spectroscopy\[J\]. J Pharm Biomed Anal, 1998, 17(1): 125-132.

[22] Moka D, Vorreuther R, Schicha H, et al. Magic angle spinning proton nuclear magnetic resonance spectroscopic analysis of intact kidney tissue samples[J]. Anal Comm, 1997, 34: 107-109.

[23] Tate A R, Foxall P J, Holmes E, et al. Distinction between normal and renal cell carcinoma kidney cortical biopsy samples using pattern recognition of ¹H magic angle spinning (MAS) NMR spectra\[J\]. NMR Biomed, 2000, 13(2): 64-71.

[24] Lucas L H, Wilson S F, Lunte C E, et al. Concentration profiling in rat tissue by high-resolution magic-angle spinning NMR spectroscopy: investigation of a model drug[J]. Anal Chem, 2005, 77(9): 2 978-2 984.

[25] Chen Wen-xue(陈文学), Deng Feng(邓风), Yue Yong(岳勇). Applicationof NMR techniques to biological tissues(核磁共振技术在生物组织中的应用)[J]. Chinese J Magn Reson(波谱学杂志), 2004, 21(1): 127-139.

[26] Cloarec O, Dumas M E, Craig A, et al. Statistical total correlation spectroscopy: an exploratory approach for latent biomarker identification from metabolic ¹H NMR data sets[J]. Anal Chem, 2005, 77(5): 1 282-1 289.

[27] Holmes E, Loo R L, Cloarec O, et al. Detection of urinary drug metabolite (xenometabolome) signatures in molecular epidemiology studies via statistical total correlation (NMR) spectroscopy\[J\]. Anal Chem, 2007, 79(7): 2 629-2 640.

[28 ]Holmes E, Cloarec O, Nicholson J K. Probing latent biomarker signatures and in vivo pathway activity in experimental disease states via statistical total correlation spectroscopy (STOCSY) of biofluids: application to HgCl₂toxicity[J]. J Proteome Res, 2006, 5(6): 1 313-1 320.

[29] Cloarec O, Campbell A, Tseng L H, et al. Virtual chromatographic resolution enhancement in cryoflow LC-NMR experiments via statistical total correlation spectroscopy[J]. Anal Chem, 2007, 79(9): 3 304-3 311.

HR-MAS ¹H NMR Spectra of Rat Kidney Tissue Analyzed by Statistical Total Correlation Spectroscopy (STOCSY)

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	LIU Wen-qing, SONG Yan-hong, WANG Xue-lu, YAO Ye-feng. In Operando Nuclear Magnetic Resonance Spectroscopy Study on Photocatalytic Methanol Reforming [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 298-308.
[2]	YIN Tian-peng, WANG Ya-rong, WANG Min, SHI Wen-zhi, ZHANG Zheng-qian, HE Sha-sha. Complete Assignments of NMR Spectral Data of Three C₁₉-Diterpenoid Alkaloids [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 331-340.
[3]	YANG Yun-han, DU Yao, YING Fei-xiang, YANG Jun-li, XIA Da-zhen, XIA Fu-ting, YANG Li-juan. Inclusion Behavior of Naringenin/β-Cyclodextrin Supramolecular Complex [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 319-330.
[4]	HU Kun, SUN Han-dong, PUNO Pema-tenzin. Application of Quantum Chemical Calculation of Nuclear Magnetic Resonance Parameters in the Structure Elucidation of Natural Products [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 359-376.
[5]	WANG Ya-lan, WANG Xiao-jing, WANG Zhi-wei. Spectral Analyses and Structural Elucidation of Azilsartan [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 350-358.
[6]	LIU Ji-hong, JIN Kun, WANG Ping, LUO Gen. An NMR Study on Esculetin and It's Derivatives [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 341-349.
[7]	WAN Zhi-bin, SONG Jian-hui, GUO Ming-ming. The Application of in Operando Liquid State NMR on Macromolecular Material Characterization [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 408-424.
[8]	TANG Ming-xue, SCHMIDT Claudia. Estimation of Nematic Order Parameters via Haller Analysis of ¹H NMR Spectra of Liquid Crystals [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 138-147.
[9]	CAO Yuan, WU Yong-ping, CHEN Dong-jun. A Spectroscopic Study on Tautomerism of Selaginellins from Selaginella [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 155-163.
[10]	TANG Heng, Gilbert NSHOGOZA, LIU Ming-qing, LIU Ya-qian, RUAN Ke, MA Rong-sheng, GAO Jia. Identification of Novel Hits of the NSD1 SET Domain by NMR Fragment-Based Screening [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 148-154.
[11]	WANG Shuang-hong, ZENG Pei, PAN Si-na, WANG Jia, WANG Shu-mei, YANG Yong-xia. Effects of Gegen Qinlian Decoction on the Fecal Metabonome of High Fructose-Induced Insulin Resistance Rats Studied by ¹H NMR Spectroscopy [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 182-194.
[12]	XIAO Xiong-jie, HU Mary, ZHANG Xu, HU Jian-zhi. An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 172-181.
[13]	XU Xiao-jun, WANG Shen-lin. Probing Membrane Protein Interactions by ¹⁹F Solid-State NMR [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 238-251.
[14]	KOU Xin-hui, LIU Yi-xiang, LIU Xing-hong, LI Cong-gang, LIU Mai-li, JIANG Ling. Visualizing the Pre-Active Conformation of Response Regulator PhoB_N^F20D in Its apo State [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 164-171.
[15]	CHEN Xiao-ying, YU Gang-jin, MAO Shi-zhen, LIU Mai-li, DU You-ru. Mixing-Induced Decreases in Critical Micelle Concentration in Aqueous Solution of Surfactants:Probing into the Mechanisms with ¹H NMR Spectroscopy [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 219-224.