Effects of Attenuated Salmonella Typhimurium Infection on Fecal Metabonome in Mice——comparison between WIPM and Bruker 500 MHz NMR spectrometers

Abstract

Abstract:

Salmonella typhimurium is a intracellular, invasive, facultative anaerobic bacteria. With improved safety, attenuated salmonella typhimurium has been widely used in development of vaccine for preventing viruses, bacteria, and parasites infections. However, limited research has been done so far on the biological effects of attenuated salmonella typhimurium infection on animal hosts. In this work, we measured fecal metabonome of mice infected with attenuated salmonella typhimurium using NMR spectroscopy. Two sets of NMR data were acquired with the same protocols on a Bruker 500 MHz NMR spectrometer and an in-house 500 MHz NMR spectrometer developed in Wuhan Institute of Physics and Mathematics (WIPM). Multivariate data analysis methods were used to analyzed the data. It was found that infection induced increases of certain amino acids and uracil in feces, and decreases of bile acids, lactate and propionate. Such metabolic alterations indicated that infection of attenuated salmonella typhimurium may cause disturbance of gut microbiotal communities. The results obtained from the home-developed spectrometer in WIPM showed high consistency with those obtained from Bruker 500 MHz NMR spectrometer, suggesting that the WIPM spectrometer is ready for metabonomic studies.

Key words: NMR, fecal metabonome, attenuated salmonella typhimurium, spectrometer

CLC Number:

O482.53

CHEN Lu1,2，SONG Kan1,3，WANG Yu-lan1*. Effects of Attenuated Salmonella Typhimurium Infection on Fecal Metabonome in Mice——comparison between WIPM and Bruker 500 MHz NMR spectrometers[J]. Chinese Journal of Magnetic Resonance.

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