Chinese Journal of Magnetic Resonance ›› 2012, Vol. 29 ›› Issue (4): 521-529.

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Chiral Recognition of  Hydroxyproline by Water-Soluble Chiral Lanthanide Shift Reagent

 LIU Yang-Lai, XU Mei-Feng, YAN Xian-Zhong*   

  1. National Center of Biomedical Analysis, Beijing 100850, China
  • Received:2012-02-03 Revised:2012-03-23 Online:2012-12-05 Published:2012-12-05
  • Supported by:

    国家科技重大专项“重大新药创制”资助项目(2009ZX09301-002,2009ZX09304-004).

Abstract:

1H  and 13C NMR spectroscopy was carried out to investigate the chiral recognition of trans-4-hydroxyproline using water-soluble chiral lanthanide shift reagent Sm-pdta. When complexed with Smpdta, most signals in the 1H  and 13C NMR spectra of trans-4-hydroxy-L-proline and trans-4-hydroxy-D-proline were shifted, leading to separation of the signals from the two enantiomers. The results showed that Sm-pdta is an effective reagent for chiral recognition of trans-4-hydroxyproline.

Key words: NMR, trans-4-hydroxyproline, Sm-pdta, chiral shift reagent, chiral recognition

CLC Number: