Chinese Journal of Magnetic Resonance

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An NMR Study on Keto-Enol Tautomerism of Indole-3-Pyruvic Acid

YANG Xiao-yan1,2,WU Xiang-yu1,2,AN Yan-peng1,2,TANG Hui-ru1*   

  1. 1. Key Laboratory of Magnetic Resonance in Biological Systems, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
    2. University of Chinese Academy of Sciences,Beijing 100049, China
  • Received:2013-05-13 Revised:2013-05-31 Online:2014-03-05 Published:2014-03-05
  • About author:*Corresponding author:TANG Hui-ru, Tel: +86-27-87198430, E-mail: huiru.tang@wipm.ac.cn.
  • Supported by:

    国家自然科学基金资助项目(21175149,21221064), 国家重点基础研究发展计划资助项目(2010CB912501).

Abstract:

Indole-3-pyruvic acid is an important α-keto acid in the tryptophan pathway. In this work, two main forms of indole-3-pyruvic acid in acetonitrile and water solutions were studied using multi-dimensional NMR techniques. The results showed that both forms were present, and the enol-form of indole-3-pyruvic acid was the dominant form in acetontrile whereas the keto-form was the main form in water. This work provided detailed assignments for the 1H and 13C NMR signals from both the enol- and keto-forms of indole-3-pyruvic acid, offering essential spectroscopic information for this metabolite.

Key words: NMR, assignment, 2D NMR, indole-3-pyruvic acid

CLC Number: