NMR-Based Metabonomic Analysis for Sulprostone-Induced Mice

Abstract

Abstract:

Prostaglandin E2 (PGE2) is a widely distributed substance in body, and involved in many physiological and pathological processes. It regulates signal transduction pathways through binding to EP1, EP2, EP3 and EP4 receptors. PGE2 is also. Sulprostone is an analogue of PGE2, playing its physiological functions through activation of EP1 and EP3. The metabolic foundation of PGE2 is still poorly understood. This work studied the metabonome of serum and liver in sulprostone-induced mice using nuclear magnetic resonance (NMR)-based analysis. The results showed that following 32 days of sulprostone treatment (i.e., 0.1 mg/kg, medium dose; 1 mg/kg, high dose), the animals showed significantly increased NADP⁺，NAD⁺，UDP，AMP and bile acids levels, and significantly reduced levels of glycogen, glucose, phenylalanine, tyrosine, uridine, inosine, nicotinic acid and GSSG in the liver. After three-weeks of recovery, choline elevation persisted in the high dose group. These results indicated that sulprostone caused metabolic disturbance of glucose, nucleic acid and amino acids in the liver. No sulprostoneinduced changes were observed in the serum data, probably due to hemeostasis. The results obtained provided more insights into the PGE2-EP1/3-signaling pathways in mouse, especially in the metabolic aspects.

Key words: NMR, sulprostone, metabonome, prostaglandin E2

CLC Number:

O482.53

YAN Xing，ZHANG Li-min，AN Yan-peng，LI Hong-de, TANG Hui-ru*. NMR-Based Metabonomic Analysis for Sulprostone-Induced Mice[J]. Chinese Journal of Magnetic Resonance.

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