Identification and Determination of Condiritul in Xiaoaiping Injection by Nuclear Magnetic Resonance Spectrometry

Abstract

Abstract:

In order to establish a quality control method for Xiaoaiping Injection, one-dimensional and two-dimensional NMR techniques used to measure the important chemical constituents, including condiritul, in the injection. An NMR method for quantitative analysis of condiritul using hydroquinone as the internal standard was presented. The proton signals of condiritul at δ 5.73 and those of hydroquinone at δ 6.73 were used for quantification. Linear regression of quantitative peak area ratio (A_x/A_s, y) of condiritul-hydroquinone versus mass ratio (W_x/W_s, x) yielded a regression equation of y=0.034 1 x+0.090 6 with a correlation coefficient of 0.999 6. The precision relative standard deviation (RSD) was 1.27%, the reproducibility RSD 2.16% and the stability RSD 0.13%. The results showed that ₁H NMR can be used for quantitative measurement of condiritul in Xiaoaiping Injection.

Key words: NMR, quantitative determination, Xiaoaiping Injection, condiritul

CLC Number:

O482.53

GAO Xiang, CHEN Dong-Jun, MA Yan-Chun, ZHU Dan-Ni, YU Bo-Yang. Identification and Determination of Condiritul in Xiaoaiping Injection by Nuclear Magnetic Resonance Spectrometry[J]. Chinese Journal of Magnetic Resonance, 2012, 29(3): 410-418.

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