Identification and Structural Determination of Saccharides in Rat Feces

Abstract

Abstract:

Fecal metabonomic NMR analysis has been found to offer important information for the complex interactions between mammalian metabolisms and their gut microbiome. In this study, we set to identify and assign saccharides signal from the NMR spectra of rat fecal samples. C18 solid-phase extraction was used to extract the saccharides from the fecal samples. The identities of the saccharides was elucidated by atomic connectivity and diffusion coefficient data obtained from 2D NMR data including ¹H-¹H COSY, ¹H-¹H TOCSY, ¹H-¹³C HSQC, ¹H-¹³C HMBC and ¹H DOSY. The saccharides identified included xylose, arabinose, galactose and arabinoxylan.

Key words: NMR, solid-phase extraction (SPE), 2D NMR, fece, oligosaccharide

CLC Number:

O482.53

TIAN Yuan, TANG Hui-Ru. Identification and Structural Determination of Saccharides in Rat Feces[J]. Chinese Journal of Magnetic Resonance, 2012, 29(3): 361-371.

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