An NMR Study on the Interaction between ATP and Biologically Active Molybdenum and Tungsten Bionic Complexes

Abstract

Abstract:

Seven molybdenum and tungsten octahedral complexes, with benzene-1,2-diol as a ligand and ethylenediamine 1, 2-diaminopropane and 1, 3-diaminopropane as counterions, respectively, were synthesized under normal temperature. The interactions between adenosinetriphosphate (ATP) and these compounds: (NH₂CH₂CH₂NH₃)₃[Mo(V)O₂(OC₆H₄O)₂] (1)，(NH₂CH₂CH₂NH₃)₂[W(VI)O₂(OC₆H₄O)₂] (2)，(NH₂CH₂CH₂NH₃)_2.5[Mo(V)_0.5W(VI)_0.5O₂(O₂C₆H₄)]₂] (3)，(NH₃CH₂CHNH₂CH₃)₃[Mo(V)O₂(OC₆H₄O)₂] (4)，(NH₃ CH₂CHNH₂CH₃)₂[W(VI)O₂(OC₆H₄O)₂] (5)，(NH₃CH₂CHNH₂CH₃)_2.5[Mo(V)_0.5W(VI)_0.5O₂(OC₆H₄O)₂] (6)，(NH₃CH₂CH₂CH₃NH₂)_2.5[Mo(V)_0.5W(VI)_0.5O₂ (OC₆H₄ O)₂] (7) were studied by LC-NMR. The results showed that the valence of the center ions were +5 when the complexes were dissolved in D₂O and the reduction of W(VI) to W(V) occurs . W(V) could be oxidized again when ATP solution was mixed with the original solution and hydrolysis of benzene-1,2-diol ligand took place.

Key words: NMR, ATP, benzene-1,2-diol, molybdenum and tungsten complexes

CLC Number:

O614. 61

BI Yan-Gang, LI Zhi-Qiang, YU Chong, SHI Xu-Dong, ZHONG Li-Juan, MIN Tao, LU Xiao-Ming. An NMR Study on the Interaction between ATP and Biologically Active Molybdenum and Tungsten Bionic Complexes[J]. Chinese Journal of Magnetic Resonance, 2010, 27(4): 562-571.

References

[1] Hille R. The mononuclear molybdenum enzymes[J]. Chem Rev, 1996, 962: 2 757-2 816.

[2] Yamase T. Polyoxometalates for molecular devices: antitumor activity and luminescence[J]. Molecular Engineering, 1993, 3: 241-262.

[3] Louis Y K, Mercouri G K, Michal S, et al. Metallocene antitumor agents. Solution and solid-state molybdencene coordination chemistry of DNA constituents[J]. J Am Chem Soc, 1991, 113: 9 027-9 036.

[4] Quintin, Kleer C G. Copper deficiency induced by tetrathiomolybdate suppresses tumor growth and angiogenesis[J]. Cancer Research, 2002, 62: 4 854-4 858.

[5] Yamase T, Ishikawa E. Proton magnetic resonance study of divalent metal ions binding to adenosine 5′-triphosphate[J]. J Inorg Biochem, 2006, 100： 344-350.

[6] Lu Xiao-ming(鲁晓明), Deng Yuan(邓元), Li Li(李丽), et al. Biomemic synthesis, crystal structure of molybdenum and tungsten chiral octahedral complex (NH₃CH₂CH₂CH₂NH₂)₂[Mo_0.4W_0.6O₂(C₆H₄O₂)₂] and its interaction with ATP by ¹H, ¹³C, ³¹P and ¹⁵N NMR(1, 3-丙二胺邻苯二酚钼钨手性八面体配合物的仿生合成、晶体结构以及与ATP作用的液相NMR研究)[J]. Chemical Journal of Chinese Universities(高等学校化学学报), 2005, 26(4): 611-616.

[7] Lu Xiao-ming(鲁晓明). The synthesis, crystal structure, EPR, UV, NMR and properties study of molybdenum and tungsten complexes(钼、钨金属配合物的合成、晶体结构、EPR、UV、NMR以及性质研究)[D]. Wuhan(武汉): Wuhan Institute of Physics and Mathematics(武汉物理与数学研究所), 2002.

[8] Lu X M, Jiang L, Mao X A, et al. Interactions with ATP, DNA cleavage and anti-tumor activities of complexes with anions[Mo(Ⅴ)O₂(O₂C₆H₄)₂]^3-, [Mo(Ⅴ)_0.5W(Ⅵ)_0.5O₂(O₂C₆H₄)₂]^2.5- and [W(Ⅵ)O₂(O₂C₆H₄)₂]^2-[J]. Chinese Journal of Chemistry, 2003, 23(6): 781-785.

[9] Song Fu-gen(宋福根), Lu Xiao-ming(鲁晓明), Yu Chong(喻冲), et al. Synthesis, crystal structure of (NH₃CH₂CHNH₂CH₃)₃[W(VI)O₂(OC₆H₄O)₂] and its NMR studies on the interaction with ATP. (NH₃CH₂CHNH₂CH₃)₃[W(VI)O₂(OC₆H₄O)₂]的合成、晶体结构及其与ATP相互作用的NMR性质研究) [J]. Chinese Journal of Chemistry(中国化学), 2009, 67(11): 1 195-1 200.

[1]	LIU Wen-qing, SONG Yan-hong, WANG Xue-lu, YAO Ye-feng. In Operando Nuclear Magnetic Resonance Spectroscopy Study on Photocatalytic Methanol Reforming [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 298-308.
[2]	YIN Tian-peng, WANG Ya-rong, WANG Min, SHI Wen-zhi, ZHANG Zheng-qian, HE Sha-sha. Complete Assignments of NMR Spectral Data of Three C₁₉-Diterpenoid Alkaloids [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 331-340.
[3]	YANG Yun-han, DU Yao, YING Fei-xiang, YANG Jun-li, XIA Da-zhen, XIA Fu-ting, YANG Li-juan. Inclusion Behavior of Naringenin/β-Cyclodextrin Supramolecular Complex [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 319-330.
[4]	HU Kun, SUN Han-dong, PUNO Pema-tenzin. Application of Quantum Chemical Calculation of Nuclear Magnetic Resonance Parameters in the Structure Elucidation of Natural Products [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 359-376.
[5]	WANG Ya-lan, WANG Xiao-jing, WANG Zhi-wei. Spectral Analyses and Structural Elucidation of Azilsartan [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 350-358.
[6]	LIU Ji-hong, JIN Kun, WANG Ping, LUO Gen. An NMR Study on Esculetin and It's Derivatives [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 341-349.
[7]	WAN Zhi-bin, SONG Jian-hui, GUO Ming-ming. The Application of in Operando Liquid State NMR on Macromolecular Material Characterization [J]. Chinese Journal of Magnetic Resonance, 2019, 36(3): 408-424.
[8]	TANG Ming-xue, SCHMIDT Claudia. Estimation of Nematic Order Parameters via Haller Analysis of ¹H NMR Spectra of Liquid Crystals [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 138-147.
[9]	CAO Yuan, WU Yong-ping, CHEN Dong-jun. A Spectroscopic Study on Tautomerism of Selaginellins from Selaginella [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 155-163.
[10]	TANG Heng, Gilbert NSHOGOZA, LIU Ming-qing, LIU Ya-qian, RUAN Ke, MA Rong-sheng, GAO Jia. Identification of Novel Hits of the NSD1 SET Domain by NMR Fragment-Based Screening [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 148-154.
[11]	XIAO Xiong-jie, HU Mary, ZHANG Xu, HU Jian-zhi. An NMR-Based Metabolomics Study of Kidneys from Mice Exposed to Ionizing Radiation [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 172-181.
[12]	XU Xiao-jun, WANG Shen-lin. Probing Membrane Protein Interactions by ¹⁹F Solid-State NMR [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 238-251.
[13]	KOU Xin-hui, LIU Yi-xiang, LIU Xing-hong, LI Cong-gang, LIU Mai-li, JIANG Ling. Visualizing the Pre-Active Conformation of Response Regulator PhoB_N^F20D in Its apo State [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 164-171.
[14]	CHEN Xiao-ying, YU Gang-jin, MAO Shi-zhen, LIU Mai-li, DU You-ru. Mixing-Induced Decreases in Critical Micelle Concentration in Aqueous Solution of Surfactants:Probing into the Mechanisms with ¹H NMR Spectroscopy [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 219-224.
[15]	WANG Shuang-hong, ZENG Pei, PAN Si-na, WANG Jia, WANG Shu-mei, YANG Yong-xia. Effects of Gegen Qinlian Decoction on the Fecal Metabonome of High Fructose-Induced Insulin Resistance Rats Studied by ¹H NMR Spectroscopy [J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 182-194.