Abstract: NMR has played an important role in Natural Product Chemistry. In this work, NMR technique was used as a valid monitor in the separation process of natural products, in addition to its application in the structural elucidation of natural compounds. In the selective separation of diterpenoid-like compounds from the natural plants, ¹H NMR was performed in time after each step of the separation. Combining with the knowledge about the spectral characteristics of the target compounds, the information obtained from ¹H NMR spectra was well used to handle the separation work.

Key words: NMR, selective separation, structural elucidation, natural product