Abstract: Two chalcones and one flavonone were isolated from the rhizomes of Ligularia vellerea: 3, 2′, 4′-trihydroxy-4-methoxy-chalcone (1), 4, 2′, 4′-trihydroxy-chalcone (2) and 7, 3′-dihydroxy-4′-methoxy flavonoe (3). The structures of these three compounds were elucidated by 1D and 2D NMR (including ¹H-¹H COSY、HSQC and HMBC). The positions of the methoxyl and the hydroxyl groups in each compound were determined.

Key words: NMR, assignment, 2D NMR, Ligularia vellerea, flavonoids