Abstract: Fluoxetine hydrochloride is a novel type of antidepressant that was developed by Eli Lilly and other companies in the United States. It has been used as a first line drug in clinical practice for treating depression, especially gerontal depression. In addition, the compound is also used for treatments of anxiety, chronic pain and eating disorders such as obesity and bulimia. Compared to the traditional tricyclic antidepressants, fluoxetine also exhibits tolerability and safety advantages. The chemical structure of fluoxetine hydrochloride is a bicyclic compound belonging to a class of selective serotonin reuptake inhibitors. In this study, the ¹H and ¹³C NMR spectra of fluoxetine were acquired, completely assigned and analyzed by a combination of 1D (¹H, ¹³C NMR and DEPT) and 2D NMR (¹H-¹H COSY，HSQC and HMBC) techniques. The results obtained in this study will provide a reference for characterization of similar compounds in the future.

Key words: NMR, assignment, 2D NMR, fluoxetine hydrochloride