Abstract: Two antibiotic compounds, actinopyrone A(1) and C (2) were separated from lyophilized culture broth of marine fungus Penicilliumsp. and their structures were elucidated with 1D and 2D NMR and HR-ESI-MS. The relative stereochemistry of compounds 1 and 2 was determined by analyzing the NMR NOESY data. Based on the NMR HMBC data, the erroneous chemical shifts of C-21 (H-21) and C-22 (H-22) reported in the literature were corrected.

Key words: NMR, structure elucidation, actinopyrone, marine fungus