Synthesis and NMR Characterization of Diethyl Puerarin-7-yl Phosphate

Abstract

Abstract:

Puerarin, an isoflavone compound, is the bioactive component of traditional Chinese medicine puerarin lobate (wild). A novel diethyl puerarin-7-yl phosphate was synthesized by the Atherton-Todd reaction with high productive yield. It was found that the compound synthesized has two sets of NMR signals at room temperature, suggesting the existence of two conformational isomers in solution. The ¹H chemical shifts of the compound were assigned using two-dimensional NMR techniques, including ¹H-detected heteronuclear multiple-quantum coherence and ¹H-detected multiple-bond heteronuclear multiple-quantum coherence.

Key words: NMR, chemical shift assignment, diethyl puerarin-7-yl phosphate, isoflavone

CLC Number:

O641

YUAN Jin-Wei, CHEN Xiao-Lan, WANG Mao-Tian, QU Ling-Bo, ZHAO Yu-Fen. Synthesis and NMR Characterization of Diethyl Puerarin-7-yl Phosphate[J]. Chinese Journal of Magnetic Resonance, 2005, 22(4): 409-414.

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