Heating of Biological Samples in Studies of MAS Solid-State NMR

doi:10.11938/cjmr20150114

Abstract

Abstract:

Magic-angle-spinning (MAS) solid-state NMR studies of biomolecules involve application of rapid sample rotation and radiofrequency pulsing, both of which can increase the sample temperature significantly, causing distorted spectra, sample dehydration and even sample degradation. In this review, the mechanisms leading to bio-sample heating in MAS solid-state NMR experiments are first introduced. The importance of sample temperature monitoring is then emphasized. Finally, we present the methods that can be used to to alleviate the problem, including optimization of sample preparation, selecting
optimal NMR parameters, and improving NMR spectrometer hardware such as the probes.

Key words: solid-state NMR, sample heating, magic angle spinning, radiofrequency

CLC Number:

O482.53

TANG Xin-qi1,2，ZHANG Zheng-feng1，YANG Jun1*. Heating of Biological Samples in Studies of MAS Solid-State NMR[J]. Chinese Journal of Magnetic Resonance, 2015, 32(1): 123-140.

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