基于核自旋单重态的活体谷胱甘肽分子MRS信号选择

doi:10.11938/cjmr20243105

摘要/Abstract

摘要：

谷胱甘肽（GSH）是一种由谷氨酸、半胱氨酸和甘氨酸聚合而成的三肽，是人体还原驱动力的主要来源，能维持人体内部氧化还原平衡．谷胱甘肽还与生物体内自由基淬灭、肿瘤治疗密切相关．利用活体磁共振波谱（MRS）监测生物体中谷胱甘肽的含量变化对于理解其生物学作用具有重要意义．由于生物体内环境复杂，活体组织的磁共振信号往往重叠严重，难以实现特定分子的选择性检测及定量．本文介绍了一种利用核自旋单重态选择性检测活体谷胱甘肽分子的方法，通过在谷胱甘肽中选择合适的自旋体系，将其制备为单重态，借助梯度脉冲消除其它分子的信号，进而实现谷胱甘肽信号的选择性检测．该方法在健康人体大脑中得到了实验验证，理论和实验结果表明，与文献中报道的其它方法相比，本文的方法具有检测效率高、选择性强的特点．

关键词: 磁共振波谱, 谷胱甘肽, 分子靶向信号检测, 核自旋单重态, 优化控制脉冲

Abstract:

Glutathione (GSH), a tripeptide derived from glutamic acid, cysteine, and glycine, as a vital source of reducing power in the body, is crucial for maintaining redox balance and combating free radicals. Its role in tumor therapy is also significant. Monitoring GSH levels in vivo using magnetic resonance spectroscopy (MRS) is essential for understanding its biological functions. However, due to complex biological environments, overlapping problem of MRS signals is often serious, which makes selective detection and accurate quantification challenging. This paper introduces a method to selectively detect GSH in vivo by using nuclear spin singlet states. Compared to other methods in literatures, our method offers high efficiency and strong selectivity.

Key words: MRS, glutathione, molecular targeted signal selection, nuclear spin singlet state, optimal control pulse

中图分类号:

O482.53

朱向炜, 杨雪, 魏达秀, 姚叶锋. 基于核自旋单重态的活体谷胱甘肽分子MRS信号选择[J]. 波谱学杂志, 2024, 41(4): 373-381.

ZHU Xiangwei, YANG Xue, WEI Daxiu, YAO Yefeng. In Vivo Glutathione Molecular MRS Signal Selection Based on Nuclear Spin Singlet States[J]. Chinese Journal of Magnetic Resonance, 2024, 41(4): 373-381.

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编号	体素大小/cm³	SFOC目标峰面积	浓度/（mmol/L）
1（水模）	2×3×2	695.46	10
2	2×3×2	81.79	1.16
3	2×3×2	86.49	1.23
4	2×3×2	56.68	0.81
5	2×3×2	96.71	1.38
6	2×3×2	86.51	1.23