1H MRS评价长期胰岛素治疗1型糖尿病大鼠海马代谢物的变化

doi:10.11938/cjmr20212955

摘要/Abstract

摘要：

本研究应用质子磁共振波谱（¹H MRS）技术对链脲佐菌素（STZ）诱导的1型糖尿病（T1DM）大鼠及长期胰岛素治疗的T1DM大鼠单侧海马的代谢物进行了分析. 结果发现，与对照组大鼠及胰岛素治疗组大鼠相比，T1DM模型组大鼠空腹血糖显著升高，体重显著降低（p < 0.05）.T1DM模型组肌醇（Ins）、牛磺酸（Tau）与谷氨酸（Glu）浓度较对照组显著升高（p = 0.000、p = 0.003、p = 0.014）.胰岛素治疗组Ins与Tau浓度较T1DM模型组显著降低（p = 0.000、p = 0.010），与对照组无差别；而Glu、谷氨酸和谷氨酰胺（Glx）浓度较对照组显著升高（p = 0.007、p = 0.042）.本文结果表明T1DM大鼠海马区代谢物Ins浓度与Tau浓度对胰岛素治疗敏感.

关键词: 质子磁共振波谱, 代谢物, 海马, 1型糖尿病, 胰岛素

Abstract:

In this study, ¹H magnetic resonance spectroscopy (¹H MRS) was used to analyze metabolites in the hippocampus of chronic type 1 diabetes mellitus (T1DM) rats induced by streptozocin and T1DM rats treated with long-term insulin. The results showed that, compared with control rats and insulin-treated T1DM rats, the fasting blood glucose of T1DM rats increased significantly, while the body weight of T1DM rats decreased remarkably (p < 0.05). The contents of myo-inositol (Ins), taurine (Tau) and glutamate (Glu) of T1DM rats were significantly higher than those of control rats (p=0.000, p=0.003, p=0.014, respectively), and the contents of Ins and Tau of insulin-treated T1DM rats were significantly decreased than those in the T1DM diabetic rats (p=0.000, p=0.010, respectively). Contents of Glu and Glx (Glu and glutamine) of insulin-treated T1DM rats were significantly higher than those in the control group (p=0.007, p=0.042, respectively). The metabolite changes of Ins and Tau in hippocampus of T1DM rats are sensitive to insulin treatment.

Key words: ¹H MRS, metabolite, hippocampus, type 1 diabetes mellitus, insulin

中图分类号:

O482.53

徐慧, 吴伊婷, 王旭霞, 康彦, 雷皓, 高丽凤. ¹H MRS评价长期胰岛素治疗1型糖尿病大鼠海马代谢物的变化[J]. 波谱学杂志, 2022, 39(4): 393-400.

Hui XU, Yi-ting WU, Xu-xia WANG, Yan KANG, Hao LEI, Li-feng GAO. Hippocampal Metabolite Alterations in Long-term Insulin-treated Type 1 Diabetes Mellitus Rats Revealed by ¹H MRS[J]. Chinese Journal of Magnetic Resonance, 2022, 39(4): 393-400.

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代谢物	CRLB/%	代谢物	CRLB/%	代谢物	CRLB/%
谷氨酸（Glu）	6.36±1.41	氮乙酰天冬氨酸（NAA）	5.6±1.26	氮乙酰天冬氨酸和氮乙酰天冬氨酸谷氨酸（tNAA）	5.64±1.26
谷胱甘肽（GSH）	12.04±3.86	牛磺酸（Tau）	7.24±2.82	肌酸和磷酸肌酸（tCr）	4.12±0.91
肌醇（Ins）	7.16±1.73	胆碱能复合物（tPC）	7.28±1.75	谷氨酸和谷氨酰胺（Glx）	6.56±1.13

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¹H MRS评价长期胰岛素治疗1型糖尿病大鼠海马代谢物的变化

Hippocampal Metabolite Alterations in Long-term Insulin-treated Type 1 Diabetes Mellitus Rats Revealed by ¹H MRS

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