NMR实验参数对IBP-血浆相互作用个性化差异研究的影响

波谱学杂志 ›› 2012, Vol. 29 ›› Issue (2): 216-223.

NMR实验参数对IBP-血浆相互作用个性化差异研究的影响

杜媛媛^1,2，纪竹生^1*，刘买利^1*

1. 波谱与原子分子物理国家重点实验室，武汉磁共振中心（中国科学院武汉物理与数学研究所），湖北武汉 430071；
2. 中国科学院研究生院，北京 100049

收稿日期:2011-05-19 修回日期:2011-05-24 出版日期:2012-06-05 发布日期:2012-06-05
基金资助:
国家自然科学基金资助项目(20975112).

Effects of NMR Parameters on Study of Individual Variations of Plasma-Ibuprofen Interactions

DU Yuan-Yuan^1,2, JI Zhu-Sheng^1*, LIU Mai-Li^1*

1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Center for Magnetic Resonance (Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China; 
2. Graduate School of the Chinese Academy of Sciences, Beijing 100049, China

Received:2011-05-19 Revised:2011-05-24 Online:2012-06-05 Published:2012-06-05
Supported by:
国家自然科学基金资助项目(20975112).

摘要/Abstract

摘要：

药物与血浆蛋白相互作用强弱是影响药物分布代谢与药效的关键因素之一. 本研究小组已报道用扩散加权谱、弛豫加权谱结合主成分分析（PCA）方法研究布洛芬（IBP）与血浆蛋白相互作用的个体差异性. 该文则研究核磁共振实验参数的设置对血浆与药物相互作用个体差异性研究的影响. 以对照血浆样品组与加入布洛芬血浆组为模型，改变扩散时间、梯度强度、回波时间这3种实验参数，采集了27套不同实验设置的扩散加权谱与10套不同回波时间的弛豫加权谱. 结果表明,扩散时间为0.1 s~0.14 s且梯度强度为1.52×10^-3T/cm~1.90×10^-3 T/cm时采集的扩散加权谱或回波时间为70 ms~110 ms时采集的弛豫加权谱更适合用来研究血浆与布洛芬相互作用的个性化差异.

关键词: 核磁共振(NMR), 个性化差异, 扩散加权谱, 弛豫加权谱, 主成分分析, 血浆, 布洛芬

Abstract:

The interactions between drug and plasma proteins play a major role in drug absorption, metabolism and its efficacy. Previous reports demonstrated that diffusion and relaxation-weighted NMR combined with principal components analysis (PCA) could be used to characterize individual variations of plasma-drug interactions. In this research, using plasma with/without drug ibuprofen (IBP) as model, the NMR parameters including diffusion time, gradient strength for diffusion-weighted NMR and echo time for relaxation-weighted NMR were optimized. The results showed that gradient strength of 1.52×10^-3T/cm~1.90×10^-3T/cm under diffusion time of 0.1 s~0.14 s for diffusion-weighted NMR and echo time of 70 ms~110 ms for relaxation-weighted NMR are optimal for characterizing individual variations of plasma-drug interactions.

Key words: nuclear magnetic resonance(NMR), individual variation, diffusion-weighted NMR, relaxation-weighted NMR, principal components analysis, plasma, ibuprofen

中图分类号:

O482.53

杜媛媛, 纪竹生, 刘买利. NMR实验参数对IBP-血浆相互作用个性化差异研究的影响[J]. 波谱学杂志, 2012, 29(2): 216-223.

DU Yuan-Yuan, JI Zhu-Sheng, LIU Mai-Li. Effects of NMR Parameters on Study of Individual Variations of Plasma-Ibuprofen Interactions[J]. Chinese Journal of Magnetic Resonance, 2012, 29(2): 216-223.

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