[1]  De Alba E,  Tjandra N. NMR dipolar couplings for the structure determination of biopolymers in solution[J]. Prog Nucl Mag Res Spectrosc, 2002，40: 175-197.

[2]  Guntert P. Structure calculation of biological macromolecules from NMR data[J]. Q Rev Biophys, 1998，31:145-237.

[3]  Karplus M. Contact electron-spin coupling of nuclear magnetic moments[J]. J  Phys  Chem,  1959，30: 11-15.

[4]  Bystrov V F. Spin-spin couplings and the conformational states of peptide spin systems[J]. Prog Nucl Magn Reson Spectrosc, 1976，10: 41-81.

[5]  Hu J S,  Bax A. Determination of phi and chi(1) angles in proteins from C¹³-C¹³  three bond J couplings measured by three-dimensional heteronuclear NMR. How planar is peptide bond?[J]. J  Am  Chem  Soc, 1997，119: 6 360-6 368.

[6]  Tjandra N. Establishing a degree of order: obtaining high-resolution NMR structures from molecular alignment[J].  Structure 1999，7:R205-211.

[7]  Prestegard J H，Al-Hashimi H M，Tolman J R,  et al. NMR structures of biomolecules using field oriented media and residual dipolar couplings[J]. Q Rev Biophys, 2000，33:371-424.

[8]  Bax A，Kontaxis G, Tjandra N.  Dipolar couplings in macromolecular structure determination[J]. Method Enzymol, 2001，339:127-174.

[9]  Saupe A. Recent results in the field of liquid crystals[J]. Angew Chem- Int  Edit  Engl,  1968，7:97-112.

[10]  Emsley J W. Liquir crystals: general considerations.  In “Encyclopedia of Nuclear Magnetic Resonance 4”[M]. (Eds: Grant D M, Harris R K)  Chichester: Wiley,  1996.

[11]  Sanders C R，Schwonek J P, et al. Characterization of magnetically orientable bilayers in mixtures of dihexanoylphosphatidylcholine and dimyristoylphosphatidylcholine by solid-state NMR[J]. Biochemistry, 1992，31:8 898-8 905.

[12]  Tjandra N，Bax A. Direct measurement of distances and angles in biomolecules by NMR in a dilute liquid crystalline medium[J].  Science, 1997， 278:1 111-1 114.

[13]  Tolman J R，Flanagan J M, Kennedy M A, et al. Nuclear magnetic dipole interactions in field-oriented proteins: information for structure determination in solution[J]. Proc Natl Acad Sci USA, 1995，92:9 279-9 283.

[14]  Contreras M A，Ubach J, Millet O, et al. Measurement of one bond dipolar couplings through lanthanide-induced orientation of a calciumbinding protein[J]. J Am Chem Soc, 1999，121:8 947-8 948.

[15]  Kung H C，Wang K Y, Goljer I, et al.  Magnetic alignment of duplex and quadruplex DNAs[J]. J Magn Reson，1995，B109:323-325.

[16]  Tjandra N，Grzesiek S，Bax A. Magnetic field dependence of nitrogen-proton J splittings in ¹⁵N-enriched human ubiquitin resulting from relaxation interference and residual dipolar coupling[J]. J  Am Chem  Soc, 1996，118:6 264-6 272.

[17]  Woessner D E. Spin relaxation processes in a two-proton system undergoing anisotropic reorirntation[J]. J Chem Phys, 1962，36:1-4.

[18]  Lipari G, Szabo A. Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity[J]. J  Am Chem  Soc, 1982，104:4 546- 559.

[19]  Lipari G,  Szabo A. Modelfree approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 2. Analysis of experimental results[J]. J Am Chem Soc,  1982，104:4 546-4 559.

[20]  Tolman J R,  Prestegard J H. Quantitative J correlation experiment for the accurate measurement of one-bond amide ¹⁵N-¹H couplings in proteins[J]. J Magn Reson, 1996，B112:245-252.

[21]  Ottiger M，Delaglio F, Bax A. Measurement of J and dipolar couplings from simplified two-dimensional NMR spectra[J].J Magn Reson,  1998，131:373-378.

[22]  Dewji N N，Wenger D A, Fujibayashi S, et al. Molecular cloning of the sphingolipid activator protein-1 (SAP-1)，the sulfatide sulfatase activator[J]. Biochem Bioph Res Commun, 1986，134:989-994.

[23]  Pervushin K，Riek R，Wider G, et al.  Attenuated T\-2 relaxation by mutual cancellation of dipole-dipole coupling and chemical shift anisotropy indicates an avenue to NMR structures of very large biological macromolecules in solution[J]. Proc Natl  Acad  Sci (USA),  1997，  94:12 366-12 371.

[24]  Yang D W，Venters R A，Mueller G A, et al. TROSY-based HNCO pulse sequences for the measurement of ¹H^N-¹⁵N，¹⁵N-¹³CO，¹H^N-¹³CO，¹³CO-¹³C^α and ¹H^N-¹³C^α dipolar couplings in ¹⁵N，¹³C，²H-labeled proteins[J]. J Biomol NMR, 1999，14:333-343.

[25]  Kontaxis G, Clore G M. Evaluation of cross-correlation effects and measurement of one-bond couplings in proteins with short transverse relaxation times[J]. J  Magn Reson, 2000，143:184-196.

[26]  Permi P,  Annila A. Transverse relaxation optimised spin-state selective NMR experiments for measurement of residual dipolar couplings
[J]. J Biomol NMR, 2000，16:221-227.

[27]  Permi P，Rosevear P R,  Annila A. A set of HNCO-based experiments for measurement of residual dipolar couplings in ¹⁵N，¹³C，²H)labeled proteins[J]. J Biomol NMR, 2000，17:43-50.

[28]  de Alba E，Suzuki M, Tjandra N. Simple multidimensional NMR experiments to obtain different types of one-bond dipolar couplings simultaneously[J]. J Biomol NMR, 2001，19:63-67.

[29]  Santoro J, King G C.  A constant-time 2D over Bodenhausen experiment for inverse correction of isotopically enriched species[J]. J Magn  Reson, 1992，97:202.

[30]  Ottiger M，Delaglio F, Marquardt J L, et al. Measurement of dipolar couplings for methylene and methyl sites in weakly oriented macromolecules and their use in structure determination[J]. J Magn  Reson, 1998，134:365-369.

[31]  Tian F，Fowler J A, Zartler E R, et al. Direct measurement of ¹H-¹H dipolar couplings in proteins: a complement to traditional NOE measurements[J]. J Biomol NMR,  2000，18:23-31.

[32]  Otting G，Ruckert M, Levitt M H, et al. NMR experiments for the sign determination of homonuclear scalar and residual dipolar couplings
[J]. J Biomol NMR, 2000，16:343-346.

[33]  Pellecchia M，Vander Kooi C W,  Keliikuli K, et al. Magnetization transfer via residualdipolar couplings: application to proton-proton correlations in partially aligned proteins[J]. J Magn  Reson, 2000，143:435-439.

[34]  Carpmagno T，Peti W,  Griesinger C.  A new method for the simultaneous measurement of magnitude and sign of 1D-{CH} and 1D-{HH} dipolar couplings in methylene groups[J]. J Biomol  NMR, 2000，17:99-109.

[35]  Sanders II C R, Landis G C. Reconstitution of membrane proteins into lipid-rich bilayered mixed micelles for NMR studies[J].    Biochemistry,  1995，34: 4 030-4 040.

[36]  Saupe A,  Englert G. High-resolution nuclear magnetic resonacnce spectra of oriented molecules[J]. Phys Rev Lett, 1963，11:462-464.

[37]  Wooton J B，Savitsky G B，Jacobus J，et al. Mehyl group geometry[J]. J Chem Phys, 1979，70:438-442.

[38]  Ottiger M, Bax A. Characterization of magnetically oriented phospholipid micelles for measurement of dipolar couplings in macromolecules
[J]. J Biomol NMR, 1998，12:361-372.

[39]  Wang H，Eberstadt M, Olejniczak E T, et al. A liquid crystalline medium for measuring residual couplingc over a wide range of temperatures[J]. J Biomol NMR, 1998，12:443-446.

[40]  Cavagnero S,  Dyson H J. Improved low pH bicelle system for orienting macromolecules over a wide temperature range[J]. J Biomol  NMR,  1999，13:387-391.

[41]  Ottiger M, Bax A. Bicelle-based liquid crystals for NMR-measurement of dipolar couplings at acidic and basic pH values[J]. J Biomol NMR,  1999，13:187-191.

[42]  Losonczi J A, Prestegard J H.  Improved dilute bicelle solutions for high-resolution NMR of biological macromolecules[J]. J Biomol NMR,  1998，12: 447-451.

[43]  Clore G M，Starich M R, Groneborn A M. Measurement of residual dipolar couplings of macromolecules aligned in the nematic phase of a colloidal suspension of rod-shaped viruses[J]. J Am Chem Soc, 1998，120:10571-10572.

[44]  Hansen M R，Mueller L, Pardi A. Tunable alignment of macromolecules by filamentous phage yields dipolar coupling interactions[J].  Nature Struc Biol,  1998，5:1 065-1 074.

[45]  Lebermann R. The isolation of plant viruses by means of simple coacervates[J]. Virology, 1966，30:341-347.

[46]  Zweckstetter M, Bax A. Characterization of molecular alignment in aqueous suspensions of Pf1 bacteriophage[J]. J Biomol  NMR，2001，  20:365-377.

[47]  Tycko R，Blanco F J, Ishii Y. Alignment of biopolymers in strained gels: A new way to create detectable dipole-dipole couplings in highresolution biomolecular NMR[J]. J Am Chem Soc, 2000，122:9 340-9 341.

[48]  Sass H J，Musco G，Stahl S J, et al. Solution NMR of proteins within polyacrylamide gels: diffusional properties and residual alignment by mechanical stress or embedding of oriented purple membranes[J]. J Biomol NMR, 2000，18:303-309.

[49]  Prosser R S，Losonczi J A, Shiyanovskaya I V.  Use of a novel aqueous liquid crystalline medium for high-resolution NMR of macromolecules in solution[J]. J Am Chem Soc, 1998，120:11 010-11 011.

[50]  Barrientos L G，Dolan C, Groneborn A M. Characterization of surfactant liquid crystal phases suitable for molecular alignment and measurement of dipolar couplings[J]. J Biomol NMR, 2000，16:329-337.

[51]  Sass H J，Cordier F，Hoffmann A, et al. Purple membrane induced alignment of biological macromolecules in the magnetic Field[J\.  J Am Chem Soc, 1999，121:2 047-2 055.

[52]  Koenig B W，Hu J S，Ottiger M, et al. NMR measurement of dipolar couplings in proteins aligned by transient binding to purple membrane fragments[J]. J Am Chem Soc, 1999，121:1 385-1 386.

[53]  Fleming K，Gray D，Prasannan S, et al. Cellulose crystallites: A new and robust liquid crystalline medium for the measurement of residual dipolar couplings[J]. J Am Chem Soc, 2000，122:5 224-5 225.

[54]  Salsbury N J，Darke A，Chapman D. Deuteron magnetic resonance studies of water associated with phospholipids[J]. Chem Phys Lipids, 1972，8:142-151.

[55]  Finer E G. Interpretation of deuteron magnetic resonance spectroscopic studies of the hydration of macromolecules[J]. J Chem Soc Farady T II, 1973，69: 1 590-1 600.

[56]  Finer E G，Darke A. Phospholipid hydration studied by deuteron magnetic resonance spectroscorpy[J]. Chem Phys Lipids, 1974，12: 1-16.

[57]  Brunner E，Ogle J，Wenzler M，et al. Molecular alignment in proteins: Quantitative evaluation of effects induced in 2D COSY spectra of proteins[J]. Biochem Biophys Commun, 2000，272: 694-698.

[58]  Hansen M R，Hanson P，Pardi A. Filamentous bacteriophage for aligning RNA，DNA and proteins for measurement of nuclear magnetic resonance dipolar coupling interactions[J]. Method Enzymol, 2000，317: 220-240.

[59]  Ramirez B E, Bax A. Modulation of the alignment tensor of macromolecules dissolved in a dilute liquid crystalline medium[J]. J Am Chem Soc, 1998，120:9 106-9 107.

[60]  De Alba E，De Vries L, Farquhar M, et al. Solution structure of human GAIP (G  interacting protein): a regulator of G protein signaling[J]. J  Mol Biol, 1999，291:927-939.

[61]  Tjandra N，Omichinski J G，Gronenborn A M, et al. Use of dipolar ¹H-¹⁵N and ¹H-¹³C couplings in the structure determination of magnetically oriented macromolecules in solution[J]. Nat Struct Biol, 1997，4:732-738.

[62]  Clore G M, Garret D S. R-factor，free R，and complete cross-validation for dipolar coupling refinement of NMR structures[J]. J Am Chem Soc, 1999，121:9 008-9 012.

[63]  Tjandra N，Marquardt J L, Clore G M. Direct refinement against proton-proton dipolar couplings in NMR structure determination of macromolecules[J]. J Magn Reson, 2000，142:393-396.

[64]  Delaglio F，Kontaxis G, Bax A. Protein structure determination using molecular fragment replacement and NMR dipolar couplings[J]. J Am Chem Soc,  2000，122:2 142-2 143.

[65]  Clore G M，Starich M R, Gronenborn A M, et al. Impact of residual dipolar couplings on the accuracy of NMR structures determined from a minimal number of NOE restraints[J]. J Am Chem Soc, 1999，121:6 513-6 514.

[66]  Nikolai R，Skrynnikov N R,  Kay L E. Assessment of molecular structure using frame-independent orientational restraints derived from residual dipolar couplings[J]. J Biomol  NMR,  2000，18:239-252.

[67]  Zweckstetter M, Bax A. Prediction of sterically induced alignment in a dilute liquid crystalline phase: Aid to protein structure determination by NMR[J]. J Am Chem Soc, 2000，122:3 791-3 792.

[68]  Losonczi J A，Andrec M，Fischer M W F, et al. Order matrix analysis of residual dipolar couplings using singular value decomposition[J].    J Magn Reson, 1999，138:334-342.

[69]  Markus M A，Gerstner R B, Draper D E, et al. Refining the overall structure and subdomain orientation of ribosomal protein S4- 41 with dipolar couplings measured by NMR in uniaxial liquid crystalline phases[J]. J Mol Biol, 1999，292:375-387.

[70]  Skrynnikov N R，Goto N K, Yang D W, et al. Orienting domains in proteins using dipolar couplings measured by liquid-state NMR: differences in solution and crystal forms of maltodextrin binding protein loaded with-cyclodextrin[J]. J Mol Biol, 2000，295:1 265-1 273.

[71]  Goto N K，Skrynnikov N R，Dahlquist F W, et al. What is the average conformation of bacteriophage T4 lysozyme in solution? A domain orientation study using dipolar couplings measured by solution NMR[J]. J Mol Biol, 2001，308:745-764.

[72]  Bolon P J，Al-Hashimi H M, Prestegard J H. Residual dipolar coupling derived orientational constraints on ligand geometry in a 53 KDa protein-ligand complex[J]. J Mol Biol, 1999，293:107-115.

[73]  Hus J C，Marion D, Blackledge M. De novo determination of protein structure by NMR using orientational and long-range order restraints[J]. J Mol Biol, 2000，298:927-936.

[74]  Mueller G A，Choy W Y, Yang D, et al. Global folds of proteins with low densities of NOEs using residual dipolar couplings: application to the 370-residue maltodextrin-binding protein[J]. J  Mol  Biol, 2000，300:197-212.