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An NMR Study on the clpC Operon Binding Region of Transcription Factor CtsR from Bacillus subtilis
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Xiao-wen CHEN,Bi-ling HUANG,Shao-hua HUANG,Yu-fen ZHAO
Chinese Journal of Magnetic Resonance, 2021, 38(2): 155-163.
DOI: 10.11938/cjmr20202854
When Gram-positive bacterium Bacillus subtilis responds to heat-shock, protein arginine kinase McsB phosphorylates Arg62 (R) in the clpC operon binding region of transcription factor CtsR, leading to dissociation of CtsR and clpC operon and initiation of stress-genes transcription for the ensuing expression of heat-shock proteins. This work investigated the clpC operon binding region (KRGGGG) of CtsR with NMR spectra (i.e., 1 H NMR, 1 H-1 H COSY, 1 H-1 H TOCSY, 1 H-15 N HSQC and 1 H-13 C HSQC). The 1 H, 13 C and 15 N chemical shifts of the protein segment were assigned. The interaction between CtsR and clpC operon and the regulatory mechanism of arginine phosphorylation were analyzed.
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Interactions Between α -synuclein and Intact Mitochondria Studied by NMR
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Jin-bo YU,Cai ZHANG,Ze-ting ZHANG,Guo-hua XU,Cong-gang LI
Chinese Journal of Magnetic Resonance, 2021, 38(2): 164-172.
DOI: 10.11938/cjmr20202841
The intrinsically disordered alpha-synuclein (α -synuclein) directly affects mitochondrial dynamics, morphology and function, and is closely related to Parkinson's disease. Studying the interactions between α -synuclein and mimic mitochondrial membranes/intact mitochondria is an effective way to understand how α -synuclein affects mitochondria. It has been reported that α -synuclein interacts with the mimic inner membrane of mitochondria (IMM), but not with the outer membrane of mitochondria (OMM), and the N-terminal plays an important role in binding with the mitochondria. However, little is known about the positions of interaction. Here, the interactions between α -synuclein and intact mitochondria isolated from rat liver were investigated by nuclear magnetic resonance (NMR). It was found that, different from the previous report, α -synuclein interacted with the OMM and the first 60 residues of N-terminus were crucial for the binding. It was postulated that the interaction between α -synuclein and OMM might depend not only on the lipid composition of the membrane, but also on other factors which were not reflected in the mimic membrane, such as the curvature of membrane and/or other components of the OMM. Our study also indicated that NMR is an effective method for studying the interaction of proteins with intact mitochondria.
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Development of a Temperature Senor Based on 19 F-labeled Phosphorylated Ubiquitin
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Zhi-wu ZHANG,Ju YANG,Ze-feng NIE,Shang-xiang YE,Xu DONG,Chun TANG
Chinese Journal of Magnetic Resonance, 2021, 38(2): 173-181.
DOI: 10.11938/cjmr20202867
Ubiquitin (Ub) is a signaling protein in eukaryotic cells, and it functions in the processes of protein degradation and DNA repairing. The published data indicated that the phosphorylation of Ub on Ser65 leads to two stable solution conformations, which can be regulated by pH. In the present project, it was found that the conformational ratio could also be modulated by temperature. Based on this feature, pUb was chemically labeled with a 19 F probe for determination of conformational ratio under various temperatures using 19 F NMR. We showed that the conformation ratio of pUb was linearly proportional to temperature gradient. Consequently, the temperature of a biological sample can be calculated with a given conformational ratio of pUb, and 19 F-labeled pUb can function as a thermosensor. This thermosensor could be used to monitor temperature of in vitro experiments, and it is also able to be taken to explore regulation of pUb function by temperature. Furthermore, the 19 F-labeled pUb thermosensor could be developed to detect intracellular temperature in-cell NMR assays.
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Image Segmentation of Tooth and Alveolar Bone with the Level Set Model
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Qin-yi SHI,Fang YAN,Yang YANG,Yue-fu CHEN,Xiao-lang LIN,Yuan-jun WANG
Chinese Journal of Magnetic Resonance, 2021, 38(2): 182-193.
DOI: 10.11938/cjmr20202827
Segmentation of tooth and alveolar bone from the cone beam computed tomography (CBCT) images provides the basic data for the three-dimensional reconstruction and visualization of bone structure. In this paper, according to the characteristics of tooth and alveolar bone, an improved potential well function was combined with the level set model for segmentation of tooth and alveolar bone, overcoming the defects of 'stop evolution' or 'too fast evolution' that might occur with the use of conventional potential well functions. Since it is difficult to effectively filter out the noises in CBCT image with the single variance Gaussian filter, a multiple small variance Gaussian filter stack was used to preprocess the image. As the contours of the same tooth in adjacent images of the image sequence showed only little changes, the segmentation result of the current layer was taken as the initial contour of the curve evolution for the next layer to reduce the times of iteration and increase the speed of segmentation. In addition, the algorithm is also used to segment a single tooth in magnetic resonance image of oral cavity successfully.
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Correction for the Nuclear Magnetic Resonance Porosity in Heavy Oil-bearing Reservoirs
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Wei ZHANG,Yi-ming WU,Wei-ping CUI,Liang XIAO
Chinese Journal of Magnetic Resonance, 2021, 38(2): 204-214.
DOI: 10.11938/cjmr20202847
The porosity acquired from nuclear magnetic resonance (NMR) logging is lower than the true value in heavy oil-bearing reservoirs due to the viscosity of heavy oil, which is a great challenge in heavy oil-bearing characterization and NMR logging. To improve the porosity prediction accuracy, the effect of heavy oil should be first corrected. In this study, 10 typical core samples, which were drilled from the Hanjiang Formation of eastern South China Sea Basin, were chosen for the laboratory NMR experimental measurements under four conditions. These four conditions were initial condition, heavy oil saturated condition, residual oil condition and water saturated condition. The effects of heavy-oil volume on NMR porosity were analyzed, and a method to estimate true NMR porosity based on formation resistivity classification was established. The method was applied in our target well A14, and the true formation porosity was predicted from field NMR logging. The result showed that, the average relative error between NMR porosity and convention core derived porosity was improved from 11.19% to 4.84% by oil viscosity correct using this method. Thus the proposed method is able to effectively correct the effect of oil viscosity on NMR porosity.
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Quantitative Evaluation of Shale Pore Structure Using Nuclear Magnetic Resonance Data
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Kun MENG,Sheng-jian WANG,Zong-an XUE,Rui-qing HOU,Liang XIAO
Chinese Journal of Magnetic Resonance, 2021, 38(2): 215-226.
DOI: 10.11938/cjmr20202856
The pore structure of shale gas reservoir is complex and heterogeneous, leading to great challenges in reservoir characterization and effectiveness evaluation. In order to establish a quantitative evaluation method for pore structure of the shale gas reservoir, 20 cores of the second member of Doushantuo Formation in Yichang area, western Hubei were selected as the experimental sample. An echo interval of 0.069 ms was used to carry out nuclear magnetic resonance (NMR) experiments under 100% salt water saturation. The obtained T 2 spectra were analyzed with the multifractal characteristics method, and the parameters sensitive to the pore structure of the shale reservoirs were extracted. A method and a standard to classify shale reservoir types were developed, based on the minimum and maximum generalized fractal dimension difference (D min -D max ) and spectral width (Δα ). This method is of great significance for improving the prediction accuracy of shale gas reservoirs and guiding development and selection.
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Preparation Efficiency of Nuclear Spin Singlet State: A Comparison Among Three Pulse Sequences
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Yi LI,Jia-xiang XIN,Jia-chen WANG,Da-xiu WEI,Ye-feng YAO
Chinese Journal of Magnetic Resonance, 2021, 38(2): 227-238.
DOI: 10.11938/cjmr20202832
The preparation and detection of the nuclear singlet state are valuable and prospective for many applications related with compound molecular analysis. In this work, a proton spin coupled system containing two H nuclei in tripeptide (Ala-Gly-Gly, AGG) was selected as research object, and singlets were prepared with three sets of different pulse sequences based on different principles. The lifetime of those singlets were measured, and the singlet preparation efficiencies of different sequences were compared. The results indicated that for the same spin coupling system of the same molecule, there is no significant difference in the lifetime of the singlet state prepared by different pulse sequences, while their preparation efficiencies could be relatively different.
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Structural Elucidation and Quantitative Analysis of Hydrogenation Products of Anthracene by NMR Spectroscopy
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Meng-yu DOU,Qi ZHAO,Xiang-lin HOU,Lei LIU,Ming-xing TANG,Ying-xiong WANG
Chinese Journal of Magnetic Resonance, 2021, 38(2): 239-248.
DOI: 10.11938/cjmr20202849
Three product mixtures of anthracene catalytic hydrogenation under different catalytic conditions were analyzed by nuclear magnetic resonance (NMR) spectroscopy. Dihydroanthracene, tetrahydroanthracene, symmetric octahydroanthracene, and asymmetric octahydroanthracene were successfully detected with diffusion-ordered spectroscopy (DOSY) and one-dimension selective gradient total correlation spectroscopy (selTOCSY) techniques. The 1 H and 13 C NMR signals of dihydroanthracene, tetrahydroanthracene and symmetric octahydroanthracene were assigned by 1 H NMR, 13 C NMR, DEPT135, 1 H-1 H COSY, 1 H-13 C HSQC. The conversion of anthracene and selectivity of the products were calculated by quantitative 1 H NMR (QNMR). The results demonstrated that NMR is a versatile and powerful tool to guide and optimize catalytic reaction conditions for increasing the selectivity of symmetrical octahydroanthracene. The present research provides a systematic NMR analysis scheme for polycyclic aromatic hydrocarbons catalytic hydrogenation.
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Nuclear Magnetic Resonance Assignment and Crystal Structure of 3, 22-Dihydroxyhopane
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Lei JIANG,Yang FU,Wen-wen GUO,Guo ZHENG,Qiang WANG
Chinese Journal of Magnetic Resonance, 2021, 38(2): 255-267.
DOI: 10.11938/cjmr20202840
Pure α - and β - isomers of 3, 22-dihydroxyhopane were obtained by the reduction of hydroxyl protected hydroxyhopanone with L -selectride. The absolute configuration of 3β , 22-hydroxyhopane was confirmed by single crystal X-ray diffraction, and the unambiguous 1 H and 13 C NMR data of two isomers were fully assigned for the first time. The preparation method is suitable for the separation of other unseparated α , β -isomers.
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Spectroscopic Studies of Delafloxacin Meglumine
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Xin-yi ZHAO,Dong HAN,Hong-jun LUO,Wen-bin SHEN,Gong-jun YANG
Chinese Journal of Magnetic Resonance, 2021, 38(2): 268-276.
DOI: 10.11938/cjmr20202855
The structure of delafloxacin meglumine was analyzed by infrared absorption (IR), ultraviolet absorption (UV), nuclear magnetic resonance (1 H NMR, 13 C NMR, DEPT, 1 H-1 H COSY, 1 H-13 C HSQC and 1 H-13 C HMBC) spectroscopy, mass spectrometry and elemental analysis. The characteristic absorption peaks in the IR spectra were discussed. The 1 H and 13 C NMR signals of delafloxacin meglumine were assigned, and the two-dimensional signals were used to elucidate its structure. The crystal structure of delafloxacin meglumine was studied by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA) and powder X-ray diffraction (PXRD).
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Conversion of Lignocellulose Studied by Nuclear Magnetic Resonance
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Xiao-li CHEN,Wei LV,Qiu-cheng SU,Juan FU,Jia-mei MO,Qi-ying LIU
Chinese Journal of Magnetic Resonance, 2021, 38(2): 277-290.
DOI: 10.11938/cjmr20202850
Nuclear magnetic resonance (NMR) is an important characterization method for the depolymerization of lignocellulosic biomass and its structure evolution analysis. The accurate NMR analysis facilitates the selective transformation of different biomass components. In this review, the principles of NMR are presented. Four types of NMR experimental methods, including 1 H NMR, 13 C NMR, 31 P NMR and 2D HSQC, and their applications in the structure elucidation, qualitative and quantitative analysis of products, reaction path and catalytic depolymerization mechanism of lignocellulose are mainly introduced. Finally, the main problems and perspectives of NMR method in valorization of lignocellulosic biomass are discussed.