An NMR Study on the clpC Operon Binding Region of Transcription Factor CtsR from Bacillus subtilis

doi:10.11938/cjmr20202854

Abstract

Abstract:

When Gram-positive bacterium Bacillus subtilis responds to heat-shock, protein arginine kinase McsB phosphorylates Arg62 (R) in the clpC operon binding region of transcription factor CtsR, leading to dissociation of CtsR and clpC operon and initiation of stress-genes transcription for the ensuing expression of heat-shock proteins. This work investigated the clpC operon binding region (KRGGGG) of CtsR with NMR spectra (i.e., ¹H NMR, ¹H-¹H COSY, ¹H-¹H TOCSY, ¹H-¹⁵N HSQC and ¹H-¹³C HSQC). The ¹H, ¹³C and ¹⁵N chemical shifts of the protein segment were assigned. The interaction between CtsR and clpC operon and the regulatory mechanism of arginine phosphorylation were analyzed.

Key words: transcription factor, nuclear magnetic resonance (NMR), chemical shifts, structure in solution

CLC Number:

O482.53

Xiao-wen CHEN,Bi-ling HUANG,Shao-hua HUANG,Yu-fen ZHAO. An NMR Study on the clpC Operon Binding Region of Transcription Factor CtsR from Bacillus subtilis[J]. Chinese Journal of Magnetic Resonance, 2021, 38(2): 155-163.

Figures/Tables 9

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Table 1

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References 12

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Position	δ_H/ppm	δ_N/ppm	δ_C/ppm
K1-α	4.07	/	52.90
K1-β	1.92	/	30.65
K1-γ	1.45	/	21.04
K1-δ	1.70	/	26.47
K1-ε	3.01	/	39.35
R2-NH-α	8.83	124.60	/
R2-α	4.38	/	/
R2-β	1.83	/	28.12
R2-γ	1.69	/	24.43
R2-δ	3.22	/	40.64
R2-NH-ε	7.18	84.72	/
G3-NH-α	8.66	111.72	/
G3-α	3.98	/	42.54
G4-NH-α	8.37/8.38	109.16/108.91	/
G4-α	4.01/4.02	/	42.54
G5-NH-α	8.37/8.38	109.16/108.91	/
G5-α	4.01/4.02	/	42.54
G6-NH-α	8.08	114.16	/
G6-α	3.83	/	43.00

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