STZ-Induced Progressive Brain Atrophy Studied by Magnetic Resonance Imaging and Histochemical Staining

doi:10.11938/cjmr20150305

Abstract

Abstract:

Type 1 diabetes mellitus (T1DM) is a chronic metabolic disease characterized by insulin deficiency. Chronic T1DM causes damages to multiple organs. Numerous cross-sectional studies have shown that T1DM patients had significant cerebral atrophy, compared to normal subjects. However, few previous studies investigated progressive changes of cerebral atrophy over time in T1DM. In this study, a rat model of T1DM was established by a single dose of intraperitoneal injection of streptozotocin (STZ). Magnetic resonance imaging (MRI) was employed to measure the volumetric changes of the brain at 12 weeks and 20 weeks after STZ induction to follow the progressive brain atrophy assessment between these two time points. Histochemical staining was used assess neuropathologic changes in the brain regions showing progressive atrophy. The MRI results demonstrated that the STZ-treated rats had significantly reduced volume of grey matter (GM), white matter (WM) and whole brain, as compared to control. Voxel-wise analysis revealed significant effect of group×time interaction in multiple GM and WM regions. Results of Nissl staining and hematein-eosin staining (HE) indicate significant neuronal abnormality in the brain regions showing progressive atrophy, including somatosensory cortex, motor cortex and hippocampal CA3 region.

Key words: MRI, type 1 diabetes, streptozotocin, histochemical staining, progressive brain atrophy

CLC Number:

O482.53

HUANG Wei1,2*，CAO Zi-yu1,2. STZ-Induced Progressive Brain Atrophy Studied by Magnetic Resonance Imaging and Histochemical Staining[J]. Chinese Journal of Magnetic Resonance, 2015, 32(3): 439-449.

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