Quantitative Analysis of Metabolites in Mungbean (Vigna Radiata) Extracts using NMR Techniques

doi:10.11938/cjmr20140409

Abstract

Abstract:

Mungbean (Vigna radiata) is an important crop that has various beneficial functions such as antioxidant and anti-inflammatory activities. However, the metabolites in mungbean have only been partially identified and quantified in previous studies. In this study, we systematically identified and assigned 48 metabolites in mungbean (Elü No.4) seeds with J-RES, COSY, TOCSY, HSQC, HMBC and 2D NMR spectra. In particular, the composition of raffinose family oligosaccharides in the extracts was determined using DOSY experiments. Most of the metabolites in the extracts were quantified. This study will provide useful information for mungbean metabolomic and nutritional studies.

Key words: nuclear magnetic resonance, assignment, 2D NMR, mungbean, quantification

CLC Number:

O482.53

WU Xiang-yu1,2，LI Ning1,2，TANG Hui-ru1*. Quantitative Analysis of Metabolites in Mungbean (Vigna Radiata) Extracts using NMR Techniques[J]. Chinese Journal of Magnetic Resonance, 2014, 31(4): 548-563.

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