Abstract: As one of the major techniques for determining high-resolution protein structures, high-field solution NMR has achieved remarkable developments during the past twenty years. With the advances of advanced spectrometer hardware, NMR pulse techniques and protein labeling strategies, the size of the protein that can be studied by solution NMR now exceeds 25 k, and can reach tens and even hundreds of kilo Dalton. In addition, solution NMR has also been widely used in the studies of protein dynamics, and is the only technology that can be used to probe protein motions at multiple sites simultaneously. In this review, we briefly summarize the methods for studying protein structures and dynamics using solution NMR, accompanied by examples to demonstrate the latest developments in the field.

Key words: NMR, protein structure, dynamics