A Metabonomic Study of Aqueous Kidney Extract in the Mouse Model of Type Ⅱ Diabetic Nephropathy

Abstract

Abstract:

An NMR-based metabonomic approach was applied to study the metabolic changes in the kidneys of db/db mice. Mice of this strain have autosomal recessive defects in the leptin receptor gene, which cause type Ⅱ diabetes and diabetic nephropathy. Metabonomic analysis in conjunction with partial least squares (PLS) analysis following orthogonal signal correction (OSC) indicated that the aqueous extract of the kidneys of db/db mice has a different metabolic profiles from that of the wild type mice, with significantly reduced levels of glutamate, acetate, choline and glycine, slight decreased levels of valine, creatinine, nicotinamide, phenylalanine and tyrosine, and significantly increased levels of lactate and sugars. The results of this study provided useful data for further understanding of type Ⅱ diabetic nephropathy.

Key words: db/db mice, metabonomic, NMR, pattern recognition

CLC Number:

O482.53

MAO Xuan, ZHANG Dong-Juan, GUAN You-Fei, YA Xian-Zhong. A Metabonomic Study of Aqueous Kidney Extract in the Mouse Model of Type Ⅱ Diabetic Nephropathy[J]. Chinese Journal of Magnetic Resonance, 2010, 27(4): 532-539.

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