Residual Dipolar Couplings and Their Applications in Determination of Protein Structures

Abstract

Abstract:

In recent years residual dipolar couplings have been employed to obtain the long-range conformational constraints such as the relative orientations between the chemical bonds in biomolecules for calculation or refinement of three-dimensional structures of proteins and protein complexes in solution. This review describes the measurement of residual dipolar couplings using multi-dimensional NMR techniques and their applications in determination of protein structures: refining protein structures, evaluating protein structures, determining the relative orientation between protein domains, obtaining information about ligand conformation and orientation, etc.

Key words: NMR, residual dipolar couplings, 3D solution structures, proteins

CLC Number:

O482.53

LIN Dong-Hai, LIU Xue-Hui. Residual Dipolar Couplings and Their Applications in Determination of Protein Structures[J]. Chinese Journal of Magnetic Resonance, 2005, 22(1): 85-98.

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