Gender Difference in Intestinal Bile Acid Profiles in C57BL/6 Mice

doi:10.11938/cjmr20182633

Abstract

Abstract: Gut microbiota is important for human health. The complex relationship between intestinal flora and bile acid profile has been studied previously. However, few previous studies have determined the profile and functions of bile acids in different intestinal segments, and whether they are related to the distribution of intestinal flora. In this paper, we measured the distribution of bile acids in different intestinal segments of male and female C57BL/6 mice with ultra-high performance liquid chromatography coupled with triple quadrupole mass spectrometry (UPLC-QqQ-MS) based targeted metabonomics analysis and nuclear magnetic resonance (NMR) based untargeted metabonomics analysis. Gender difference in distributions of bile acids were observed, which was more evident in the lower parts of intestine than in the upper part of intestine, especially in the cecum segment. This might be related to the abundance of gut microflora in different intestine segments. This study provided metabolic phenotypes of bile acids in mice of different genders, and demonstrated their interactions with gut microbiota.

Key words: metabonomics, bile acid, C57BL/6 mice, gender difference, gut microbiota

CLC Number:

O482.53

ZHANG Cong-cong, WU Jun-fang, WANG Yu-lan. Gender Difference in Intestinal Bile Acid Profiles in C57BL/6 Mice[J]. Chinese Journal of Magnetic Resonance, 2018, 35(3): 328-337.

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