A New Gd-Based T2-Weighted Magnetic Resonance Imaging Contrast Agent:Preparation and Application in Stem Cell Imaging

doi:10.11938/cjmr20172558

Abstract

Abstract: We synthesized TPP-Lys(Acp-DOTA-Gd)-COOH (Gd-DOTA-TPP) as a novel T₂-weighted MRI contrast agent, and used it to label human mesenchymal stem cells (hMSCs) via electroporation. hMSCs labeled with Gd-DOTA-TPP presented a rather dark T₂-weighted image at a cellular Gd content of about 9×10⁹ Gd/cell. The labeled cells were syringe-injected into a mouse brain with defined cell numbers followed by T₂-weighted MRI on an 11.7 T system, which yield an in vivo imaging sensitivity of about 10³ cells.

Key words: contrast agent, human mesenchymal stem cells (hMSCs), magnetic resonance imaging (MRI), TPP-Lys(Acp-DOTA-Gd)-COOH (Gd-DOTA-TPP), sensitivity

CLC Number:

O482.53

ZHANG Yan-hui, ZHANG Hong-yan, ZHANG Hai-lu, ZHANG Peng-li, JIANG Hai-zhen, DENG Zong-wu, TAN Bo. A New Gd-Based T₂-Weighted Magnetic Resonance Imaging Contrast Agent:Preparation and Application in Stem Cell Imaging[J]. Chinese Journal of Magnetic Resonance, 2017, 34(3): 302-310.

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A New Gd-Based T₂-Weighted Magnetic Resonance Imaging Contrast Agent:Preparation and Application in Stem Cell Imaging

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