A Magnetic Resonance-Fluorescent Dual-Mode Imaging Probe for Stem Cell Tracking

doi:10.11938/cjmr20182659

Abstract

Abstract: A magnetic resonance (MR)-fluorescent dual-mode imaging probe was prepared by conjugating guaiazulene to Gd-DOTA, and used to label human mesenchymal stem cells (hMSCs) via electroporation. The probe self-assembled into nanoclusters in the cytoplasm, resulting in a significant reduction in T₂-weighted signal intensity that persisted up to 7 days. The fluorescence signal of the probe was observed at 498 nm, and the labelled hMSCs emitted a green fluorescence. The MR-fluorescent dual-mode imaging probe can potentially be used for stem cell tracking in vivo.

Key words: magnetic resonance imaging, fluorescent imaging, dual-mode probe, human mesenchymal stem cell

CLC Number:

O482.53

ZHAO Min-min, ZHANG Yan-hui, ZHANG Peng-li, TAN Bo, JIANG Hai-zhen, ZHANG Hai-lu, DENG Zong-wu. A Magnetic Resonance-Fluorescent Dual-Mode Imaging Probe for Stem Cell Tracking[J]. Chinese Journal of Magnetic Resonance, 2019, 36(2): 127-137.

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