Chinese Journal of Magnetic Resonance

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Differentiating Bupleurum bicaule from Bupleurum chinense Using 1H NMR Spectroscopy

SONG Ai-hua1,SHA Yi1,XU Xiao-xue2,LI Wen1,XU Nan2*,LI Xiao-peng2,HAN Wei-jian2,LIN Xiao-tong2

 
  

  1. 1. Testing center, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China
  • Received:2013-07-08 Revised:2013-07-26 Online:2014-06-05 Published:2014-06-05
  • About author:*Corresponding author:XU Nan, Tel: 0411-87586014; E-mail: xudanbs@163.com.
  • Supported by:

    国家公益性行业专项课题资助项目(201007007).

Abstract:

Bupleuuri Radix(Chaihu in Chinese)is a widely utilized traditional Chinese medicine for soothing liver and regulating vital energy. According to Chinese pharmacopoeias,only the roots of Bupleurum chinense are considered to be Bei Chaihu. In practice, However, roots of other Bupleurum plants, such as Bupleurum bicaule, are also sold or used as Bei Chaihu. In order to find to analytical tool to differentiate the roots of Bupleurum chinense and Bupleurum bicaule, we used 1H NMR to analyze the composition of secondary metabolites of the two species. It was found that the metabolic fingerprint of the Bupleurum chinense is different from that of the Bupleurum bicaule, and thus 1H NMR can be used to differentiate the two.

Key words: 1H NMR, Bupleuri Radix, PLS-DA, secondary metabolites

CLC Number: