Changes of Chemical Constitution in Alismatis rhizoma after Saline Processing as Revealed by 1H NMR Analysis

doi:10.11938/cjmr20160111

Abstract

Abstract:

As a commonly used traditional Chinese medicine (TMC), Alismatis rhizoma is the main ingredient of many classical formulas, such as Wu Ling San, Long Dan Xie Gan Wan and Liu Wei Di Huang Wan. Both the raw slices of Alismatis rhizoma and the slices processed with saline arelisted are prescription drugs in the Pharmacopoeia of People's Republic of China. TMC theory thinks the raw and processed slices of Alismatis rhizoma are different in function. The raw slices are emphasized on its actions of alleviating water retention and dispersing heat, whiles the processed slices are thought ineffective in these respects. The active components in these two types of Alismatis rhizoma slices remained poorly understood, making standardizing the saline processing procedure and quality control difficult. In this paper, we applied ¹H NMR based metabonomic approach to investigate the difference of chemical constitution between the raw and salt-processed slices of Alismatis rhizome. The result showed that the chemical constitution of the Alismatis rhizoma slices changed significantly after salt-processing, and such changes were influenced by the length of processing time. In PLS-DA analysis, the signals of alisols at δ 3.82, 3.78, 3.66, 1.34, 0.94, and 1.30 changed significantly under different processing conditions, indicating that alisols were perhaps the chemical fingreprints contributing the different functions of raw and salt-processed Alismatis rhizoma slices.

Key words: ¹H NMR, Alismatis rhizoma, metabonomic analysis, processing, chemical constitution

CLC Number:

O482.53

HAN Wei-jian, LIN Xiao-tong, GUO Na, CHEN Qiao, ZHANG Ting-ting, XU Nan. Changes of Chemical Constitution in Alismatis rhizoma after Saline Processing as Revealed by ¹H NMR Analysis[J]. Chinese Journal of Magnetic Resonance, 2016, 33(1): 117-124.

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Changes of Chemical Constitution in Alismatis rhizoma after Saline Processing as Revealed by ¹H NMR Analysis

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