MRG15与甲基化组蛋白H3K36相互作用研究

波谱学杂志 ›› 2012, Vol. 29 ›› Issue (4): 598-604.

MRG15与甲基化组蛋白H3K36相互作用研究

洪晶¹，刘华¹，俞世宏¹，陈涛涛¹，林东海^2*

1. 福州大学生物科学与工程学院，福建福州 350002; 2. 厦门大学化学化工学院，福建厦门 361005

收稿日期:2012-09-13 修回日期:2012-09-28 出版日期:2012-12-05 发布日期:2012-12-05
基金资助:
福建省教育厅资助项目（JA11019）；福建大学科技发展基金资助项目(2010-XQ-18).

Interaction between MRG15 and Methylated Histone H3K36 Studied by NMR Spectroscopy

HONG Jing¹, LIU Hua¹, YU Shi-Hong¹, CHEN Tao-Tao¹, LIN Dong-Hai^2*

1. College of Biological Science and Technology, Fuzhou University, Fuzhou 350002, China;
2. College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Received:2012-09-13 Revised:2012-09-28 Online:2012-12-05 Published:2012-12-05
Supported by:
福建省教育厅资助项目（JA11019）；福建大学科技发展基金资助项目(2010-XQ-18).

摘要/Abstract

摘要：

人源转录调控因子MRG15（MORF4 related gene on chromosome 15）是在胚胎发育，细胞增殖和衰老过程中具有重要作用的蛋白质. 在分子水平上，MRG15的chromo结构域能够与组蛋白H3甲基化的肽段H3K36me3相互作用. 为了确定二者的作用位点和方式，利用液体核磁共振的方法对MRG15 chromo结构域和H3K36me3多肽进行了研究. 对MRG15 chromo结构域进行归属，用化学位移扰动的方法研究了其与H3K36me3肽段的相互作用，确定了相互作用位点的关键氨基酸为H21，Y46，W49和W53，并计算了解离常数约为1 mmol/L. 研究结果对于阐明MRG15与组蛋白H3的结合和相互作用机制提供了重要的线索.

关键词: 核磁共振(NMR), 相互作用, HSQC, MRG15, 化学位移扰动

Abstract:

Human MRG15 is a transcription factor that plays an important role in embryonic development, cell proliferation and senescence. The MRG15 chromo domain can interact with methylated Lys36 of histone H3. In this study, the assignment of amino acids of MRG15 chromo domain was done using solution NMR spectroscopy. The interaction between MRG15 chromo domain and H3K36me3 peptide was studied by the chemical shift perturbation approach. The binding sites (i.e., H21，Y46，W49 and W53) and dissociation constants (~1 mmol/L) were determined. These results provide give insight on the interaction between MRG15 chromo domain and histone H3 at molecular level.

Key words: NMR, protein interaction, HSQC, MRG15, chemical shift perturbation

中图分类号:

Q518.2

洪晶, 刘华, 俞世宏, 陈涛涛, 林东海. MRG15与甲基化组蛋白H3K36相互作用研究[J]. 波谱学杂志, 2012, 29(4): 598-604.

HONG Jing, LIU Hua, YU Shi-Hong, CHEN Tao-Tao, LIN Dong-Hai. Interaction between MRG15 and Methylated Histone H3K36 Studied by NMR Spectroscopy[J]. Chinese Journal of Magnetic Resonance, 2012, 29(4): 598-604.

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