Ⅱ型糖尿病肾病小鼠肾水提物代谢组学研究

波谱学杂志 ›› 2010, Vol. 27 ›› Issue (4): 532-539.

• 恭贺沈联芳和杜有如两位先生八十华诞专栏 • 上一篇下一篇

Ⅱ型糖尿病肾病小鼠肾水提物代谢组学研究

毛璇¹，张冬娟²，管又飞²，颜贤忠^1*

1. 国家生物医学分析中心, 北京 100850； 2. 北京大学医学部生理学与病理生理学系, 北京 100083

收稿日期:2010-04-07 修回日期:2010-05-01 出版日期:2010-12-05 发布日期:2010-12-05
基金资助:
国家自然科学基金资助项目（90409019），国家科技重大专项资助项目(2008ZX10002-016).

A Metabonomic Study of Aqueous Kidney Extract in the Mouse Model of Type Ⅱ Diabetic Nephropathy

MAO Xuan¹, ZHANG Dong-Juan², GUAN You-Fei², YA Xian-Zhong^1*

1. National Center of Biomedical Analysis, Beijing 100850, China;
2. Department of Physiology and Pathophysiology, Peking University, Beijing 100083, Chin

Received:2010-04-07 Revised:2010-05-01 Online:2010-12-05 Published:2010-12-05
Supported by:
国家自然科学基金资助项目（90409019），国家科技重大专项资助项目(2008ZX10002-016).

摘要/Abstract

摘要：

应用基于核磁共振的代谢组学方法研究Ⅱ型糖尿病模型db/db小鼠已发展到糖尿病肾病时肾脏代谢表型的变化. 结合偏最小二乘法-判别分析（PLS-DA），结果显示，与正常组相比，模型组肾水提物中的乳酸和糖等代谢物的含量显著升高，而谷氨酸、乙酸、胆碱和甘氨酸的含量显著降低，缬氨酸、肌酐、尼克酰胺、苯丙氨酸和酪氨酸含量略有降低. 实验结果表明糖尿病肾病动物模型db/db小鼠的代谢特征不同于正常小鼠. 代谢组学分析方法作为辅助手段，为糖尿病肾病的发病机制提供了良好的数据支持.

关键词: 核磁共振(NMR), db/db小鼠, 代谢组学, 模式识别

Abstract:

An NMR-based metabonomic approach was applied to study the metabolic changes in the kidneys of db/db mice. Mice of this strain have autosomal recessive defects in the leptin receptor gene, which cause type Ⅱ diabetes and diabetic nephropathy. Metabonomic analysis in conjunction with partial least squares (PLS) analysis following orthogonal signal correction (OSC) indicated that the aqueous extract of the kidneys of db/db mice has a different metabolic profiles from that of the wild type mice, with significantly reduced levels of glutamate, acetate, choline and glycine, slight decreased levels of valine, creatinine, nicotinamide, phenylalanine and tyrosine, and significantly increased levels of lactate and sugars. The results of this study provided useful data for further understanding of type Ⅱ diabetic nephropathy.

Key words: db/db mice, metabonomic, NMR, pattern recognition

中图分类号:

O482.53

毛璇, 张冬娟, 管又飞, 颜贤忠. Ⅱ型糖尿病肾病小鼠肾水提物代谢组学研究[J]. 波谱学杂志, 2010, 27(4): 532-539.

MAO Xuan, ZHANG Dong-Juan, GUAN You-Fei, YA Xian-Zhong. A Metabonomic Study of Aqueous Kidney Extract in the Mouse Model of Type Ⅱ Diabetic Nephropathy[J]. Chinese Journal of Magnetic Resonance, 2010, 27(4): 532-539.

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Ⅱ型糖尿病肾病小鼠肾水提物代谢组学研究

A Metabonomic Study of Aqueous Kidney Extract in the Mouse Model of Type Ⅱ Diabetic Nephropathy

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