关键词: 蛋白质, 核磁共振, 支链, 动力学, 弛豫

Abstract:

Dynamic aspects of proteins are increasingly recognized as being important for describing their biological functions such as protein-protein and protein-drug interactions. Consequently, considerable efforts have been made to characterize protein dynamic motions. Among these methods, nuclear magnetic resonance (NMR), in particular, is capable of providing valuable information on the nature of internal motions of proteins in solution. In this review, the NMR-based approaches for studying protein side chain dynamics are reviewed, with emphasis on those methods for probing fast time scale internal motions.

Key words: NMR, protein, side chain, dynamics, relaxation