波谱学杂志 ›› 2005, Vol. 22 ›› Issue (1): 113-121.

• 综述评论 • 上一篇    

31P和19F NMR在细胞内游离镁离子测定中的研究

  

  1. 军事医学科学院 放射医学研究所, 北京 100850
  • 收稿日期:2004-06-24 修回日期:2004-08-23 出版日期:2005-03-05 发布日期:2005-03-05
  • 基金资助:

    国家自然科学基金资助项目(30271608); 北京市自然科学基金资助项目(7042048).

Noninvasive Determination of Intracellular Free Mg2+ by 31P and 19F NMR: A Review

  1. Institute of Radiation Medicine,  Academy of Military Medical Sciences,  Beijing 100850,  China
  • Received:2004-06-24 Revised:2004-08-23 Online:2005-03-05 Published:2005-03-05
  • Supported by:

    国家自然科学基金资助项目(30271608); 北京市自然科学基金资助项目(7042048).

摘要:

目前有多种分析方法可以测定生物细胞内镁离子的浓度,但这些方法都有一定的弊端,需要将细胞破坏,而且不能进行连续的测定. 核磁共振(NMR)方法是一种可以对活细胞、组织等进行无损伤的测定的技术,允许在较长时间内连续进行观察,可以同时考察胞内、胞外离子浓度. 核磁共振测定生物细胞内镁离子的31P NMR 和19F NMR方法近年来多应用于临床医学、生物分析等方面. 

关键词: 31P NMR,  19F NMR, 游离镁离子, 无损伤测定

Abstract:

Magnesium ion (Mg2+) plays important roles in many biochemical reactions occurring in cells,  and determination of intracellular free Mg2+ concentration is essential for understanding the functioning of intact cells. Nuclear magnetic resonance (NMR),  a spectroscopic technique that measures magnetic nuclei and their interactions with the environment,  has gained considerable success and popularity in studying metal ions in vivo. 31P and 19F NMR can be used to measure intracellular concentration of free Mg2+,  and these techniques has the advantage of noninvasiveness so that free Mg2+ in the intracellular environment can be observed in essentially unperturbed living systems. In this review,  the theories and applications of 31P  and 19F NMR for determining intracellular concentration of free Mg2+ are discussed:.

Key words: 31P NMR,  19F NMR,  Mg2+,  noninvasive determination

中图分类号: